确定与持续疼痛相关的应对行为所需的途径
Tianwen Huang1,2, Shing-Hong Lin1,2, Nathalie M Malewicz3
1Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA.
Nature
|December 12, 2018
概括
研究人员在小鼠中发现了特定的脊髓神经元, 这一发现揭示了持续疼痛反应的神经基础,
科学领域:
- 神经科学
- 疼痛研究
- 人体感官系统
背景情况:
- 有害刺激会引起动物和人类不同的反射和应对反应.
- 这些双重疼痛反应背后的神经回路尚未完全理解.
- 了解这些循环对于疼痛管理和研究至关重要.
研究的目的:
- 确定负责疼痛应对行为的特定脊髓神经回路.
- 研究TAC1表达脊髓神经元在中介持续疼痛反应中的作用.
- 在疼痛处理中探索脊柱电路与中部质核之间的关系.
主要方法:
- 使用遗传工具 (TAC1Cre和LBX1Flpo) 向和切除小鼠的特定脊髓神经元.
- 评估对有害刺激的行为反应,包括,厌恶和反射性退缩.
- 研究了从脊髓到中枢的神经突出.
- 研究了特定的主感神经元 (MRGPRD和TRPV1) 在不同疼痛反应中的作用.
主要成果:
- 切除协同表达TAC1和LBX1的脊髓神经元,消除了持续的和对持续疼痛刺激的条件反感.
- 这些特定的脊髓神经元直接或间接地投射到中枢乳头核.
- 经过切除后,反射性防御反应保持不变,这表明在应对过程中具有选择性作用.
- 主要感官神经元 (MRGPRD和TRPV1) 显示反射与应对疼痛行为的功能分离.
结论:
- 共同表达TAC1和LBX1的脊髓神经元是痛苦应对行为的关键驱动因素.
- 一个独特的神经通路涉及脊柱TAC1系神经元和中间丘脑,调解持续的疼痛反应.
- 这些发现揭示了不同疼痛行为体感系统的基本细分.
- 目前仅依靠反射反应的方法可能无法完全捕捉持续疼痛的体验.
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