一个通过种子转向无性繁殖的雄性表达的米胚胎触发
Imtiyaz Khanday1,2, Debra Skinner1, Bing Yang3
1Department of Plant Biology, University of California, Davis, CA, USA.
Nature
|December 14, 2018
概括
在植物中,受精需要精子细胞的特定转录因子,如BBM1. 这种基因可以诱导无性繁殖 (生殖) 并使杂交作物的克隆繁殖.
科学领域:
- 植物生殖生物学
- 分子遗传学
- 发育生物学
背景情况:
- 在开花植物中控制受精依赖的胚胎发生的分子机制在很大程度上是未知的.
- 了解这些途径对于植物育种和作物改进至关重要.
研究的目的:
- 调查Baby Boom1 (BBM1) 在启动植物胚胎发生的作用及其无性繁殖的潜力.
- 探索克隆繁殖杂交作物的可行性.
主要方法:
- 在大米 (Oryza sativa) 中研究了AP2转录因子BBM1的功能.
- 在卵细胞和基因组编辑技术 (MiMe) 中利用BBM1的子宫外表达.
- 对BBM1,BBM2和BBM3进行了基因淘汰实验.
主要成果:
- 在卵细胞中诱导BBM1的子宫外表达,绕过受精.
- 通过男性传播的BBM1在三重BBM淘汰突变中挽救了胚胎发育.
- 将BBM1表达与MiMe基因组编辑相结合,产生了可活的异构细胞后代.
结论:
- 像BBM1这样的多能性因子的男性基因组传播介导了胚胎生成的受精要求.
- 在作物中无性繁殖是可行的,可以通过种子繁殖来维持杂交特征的克隆.
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