在双细胞小鼠胚胎中LincGET偏差细胞命运的不对称表达
Jiaqiang Wang1, Leyun Wang2, Guihai Feng2
1State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, China; University of Chinese Academy of Sciences, 100049 Beijing, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, 100101 Beijing, China; College of Life Science, Northeast Agricultural University, 150030 Harbin, China.
Cell
|December 15, 2018
概括
一种新的长非编码RNA,LincGET,在哺乳动物胚胎中起到早期的调节作用. 它通过与CARM1相互作用,影响基因表达和染色质的可访问性来偏向细胞命运.
科学领域:
- 发育生物学
- 表观遗传学
- 基因组学
背景情况:
- 早期哺乳动物胚胎的初始细胞命运决定对发育至关重要,但仍然不太清楚.
- 了解驱动早期细胞命运分离的机制是解读胚胎发育的关键.
研究的目的:
- 研究长非编码RNA在哺乳动物胚胎中早期细胞命运偏差的作用.
- 确定细胞内质量 (ICM) 谱系规范的新型调节剂.
主要方法:
- 在小鼠胚胎中的长非编码RNA LincGET的表达分析.
- 功能性研究涉及LincGET和CARM1在胚芽细胞中的过度表达和耗尽.
- 染色体免疫沉和基因表达分析以评估表观遗传修饰和基因激活.
主要成果:
- 在早期小鼠胚胎中,LincGET是暂时且不对称的表达.
- 过度表达的LincGET会使胚芽细胞后代偏向ICM命运.
- LincGET与CARM1相互作用,促进H3R26me,ICM基因表达,对转位子的上调和染色质的可访问性.
结论:
- LincGET被认为是哺乳动物胚胎细胞命运的早期调节者.
- LincGET对ICM命运的基因偏差影响取决于CARM1.
- 这项研究揭示了一种控制早期细胞命运的新型表观遗传机制.
相关概念视频
Confirmation Biases
8.2K
The confirmation bias is the tendency to focus on information that confirms our existing beliefs and ignore information that is inconsistent with our expectations. For example, if you think that your professor is not very nice, you notice all of the instances of rude behavior exhibited by the professor while ignoring the countless pleasant interactions he is involved in on a daily basis. Have you ever fallen prey to the confirmation bias, either as the source or target of such bias?
8.2K
Cell Specific Gene Expression
16.5K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.5K
Cell Specific Gene Expression
5.6K
5.6K
Hindsight Biases
4.3K
Hindsight bias leads you to believe that the event you just experienced was predictable, even though it really wasn’t. In other words, you knew all along that things would turn out the way they did. Can you relate this to the phrase "Hindsight is 20/20" now?
4.3K
Bias
7.4K
Bias refers to any tendency that prevents a question from being considered unprejudiced. In research, bias occurs when one outcome or answer is selected or encouraged over others in sampling or testing. Bias can occur during any research phase, including study design, data collection, analysis, and publication.
In statistics, a sampling bias is created when a sample is collected from a population, and some members of the population are not as likely to be chosen as others (remember, each member...
In statistics, a sampling bias is created when a sample is collected from a population, and some members of the population are not as likely to be chosen as others (remember, each member...
7.4K
Fates of Pyruvate
10.8K
Pyruvate is the end product of glycolysis, where glucose is oxidized to pyruvate, simultaneously reducing NAD+ to NADH. Two molecules of ATP are also produced by substrate-level phosphorylation.
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
10.8K


