在植入前对小鼠胚胎进行调节
Anna Hupalowska1, Agnieszka Jedrusik1, Meng Zhu1
1Department of Physiology, Development and Neuroscience, University of Cambridge, Downing Street, Cambridge CB2 3EG, UK.
Cell
|December 15, 2018
概括
早期小鼠胚胎的发育依赖于核颗粒和. 这些结构涉及CARM1和Neat1,调节细胞系的建立和分化,影响胚胎和胚胎外的命运.
科学领域:
- 细胞生物学
- 发育生物学
- 表观遗传学
背景情况:
- 核架构对于早期哺乳动物的发展和血统规范至关重要.
- 甲基转移酶CARM1和基因素H3R26的甲基化涉及到建立胚胎和非胚胎血统.
- 在这些早期发育过程中,核颗粒和粒的作用在很大程度上仍未被探索.
研究的目的:
- 研究核颗粒在小鼠胚胎早期发育中的作用.
- 为了阐明CARM1,和基因组甲基化在血统建立之间的关系.
- 了解在细胞命运确定过程中对光和CARM1的上游或下游功能.
主要方法:
- 免疫光显微镜观察小鼠胚胎中的CARM1局部化.
- 使用siRNA击败Neat1和p54nrb的耗尽研究.
- 基因组H3R26甲基化水平的分析.
- 评估Cdx2表达和发育进展.
主要成果:
- 在小鼠胚胎的2至4细胞转化阶段,CARM1局部化为核颗粒,主要是粒.
- Neat1和p54nrb对于CARM1的光谱协会和H3R26甲基化是必不可少的.
- Neat1或p54nrb的耗尽导致16至32个细胞阶段的发育停止,增加了Cdx2的表达,这表明偏向于胚胎外血统分化.
- 另外,CARM1也会影响镜组织,这表明它们之间存在相互关系.
结论:
- 通过Neat1和p54nrb,对CARM1介导的H3R26甲基化和适当的血统建立至关重要.
- 在调节发育进展和细胞命运决策方面,寄光素在CARM1上游起作用.
- 这些发现揭示了虫在早期哺乳动物发育过程中协调核组织和表观遗传修饰的新角色.
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