宿主产生的定数感应自导器控制菌体溶解-溶解决定
Justin E Silpe1, Bonnie L Bassler2
1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Cell
|December 18, 2018
概括
霍乱病毒及其菌体使用相同的信号分子DPO进行致病. 菌体VqmA激活宿主病毒性基因,而宿主VqmA抑制它们,揭示了细菌感染中的复杂相互作用.
科学领域:
- 微生物学
- 分子生物学
- 细菌学
背景情况:
- 霍乱病毒利用含有3,5-二甲基-2-醇 (DPO) 和VqmA (VqmAVc) 的定数感应 (QS) 系统来调节毒性和生物膜的形成.
- 该vqmA基因在Vibrio物种和食者VP882中发现,这表明水平基因转移和共享的调节机制.
研究的目的:
- 研究菌体编码的VqmA (VqmA菌体) 在V. cholerae及其感染菌体之间的相互作用中的作用.
- 了解菌体如何将宿主衍生的信号整合到它们的生命周期决策中 (溶解-溶解).
- 探索重新编程菌体以控制细菌感染的可能性.
主要方法:
- 对VqmAVc和VqmAPhage与DPO的相互作用进行比较分析.
- 检测菌体抗抑制剂的功能及其与相关菌体抑制剂的干扰.
- 用于评估宿主VqmAVc regulon被VqmAPhage激活的功能测试
- 重编程菌体以响应用户定义的溶解诱导线索.
主要成果:
- 菌体VqmA菌体与宿主产生的DPO结合,启动菌体溶解程序.
- VqmAPhage通过抗抑制剂作用,使菌体抑制剂失活并干扰相关的菌体抑制剂.
- VqmAPhage激活宿主VqmAVc规律子,显示出一个不对称的调节相互作用.
- 主体VqmAVc不能诱导菌体介导的溶解,突出显示菌体的单向影响.
结论:
- 霍乱病毒及其感染菌体都依赖于相同的信号分子 (DPO) 致病.
- 菌体利用宿主QS机制进行自身的繁殖,将宿主信号整合到溶解-溶解生成决策中.
- 重新编程菌体为治疗细菌感染提供了一个潜在的策略.
相关概念视频
Gene Regulation in Microbial Communities: Quorum Sensing
597
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
597
The Sense of Self: Reflected Self-Appraisal and Social Comparison
56.1K
According to Charles Cooley, we base our image on what we think other people see (Cooley 1902). We imagine how we must appear to others, then react to this speculation. We don certain clothes, prepare our hair in a particular manner, wear makeup, use cologne, and the like—all with the notion that our presentation of ourselves is going to affect how others perceive us. We expect a certain reaction, and, if lucky, we get the one we desire and feel good about it. But more than that, Cooley...
56.1K
Decision Making
970
Decision-making is a fundamental cognitive process that involves evaluating alternatives and selecting among them. This process can range from simple choices, such as deciding what to wear, to complex decisions, like choosing a major in college or a career path. The complexity of the decision often dictates the approach we use, which can be broadly categorized into two types: automatic and controlled decision-making.
Automatic decision-making is fast, intuitive, and relies on gut feelings...
Automatic decision-making is fast, intuitive, and relies on gut feelings...
970
Tissue Homogenization and Cell Lysis
10.2K
Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...
10.2K
Introduction to Special Senses
7.5K
Sensory receptors play an integral part in comprehending our external and internal environments. They receive diverse stimuli, converting them into the nervous system's electrochemical signals. This conversion occurs as the stimulus alters the sensory neuron's cell membrane potential, instigating the generation of an action potential. This action potential is subsequently transmitted to the central nervous system (CNS), which integrates with other sensory data or higher cognitive...
7.5K
Decision Making: P-value Method
6.9K
The process of hypothesis testing based on the P-value method includes calculating the P- value using the sample data and interpreting it.
First, a specific claim about the population parameter is proposed. The claim is based on the research question and is stated in a simple form. Further, an opposing statement to the claim is also stated. These statements can act as null and alternative hypotheses: a null hypothesis would be a neutral statement while the alternative hypothesis can...
First, a specific claim about the population parameter is proposed. The claim is based on the research question and is stated in a simple form. Further, an opposing statement to the claim is also stated. These statements can act as null and alternative hypotheses: a null hypothesis would be a neutral statement while the alternative hypothesis can...
6.9K


