抗氧化光蓝化的三甲
Alexey N Butkevich1,2, Mariano L Bossi2, Gražvydas Lukinavičius1
1Department of NanoBiophotonics , Max Planck Institute for Biophysical Chemistry , Am Fassberg 11 , 37077 Göttingen , Germany.
Journal of the American Chemical Society
|December 19, 2018
概括
我们开发了新的罗达胺光体, 这些探测器抵御光漂白和光转换,提高了超分辨率显微镜中的数据可复制性.
科学领域:
- 化学合成和光物理
- 光显微镜和光谱学
- 用于成像探测器的材料科学
背景情况:
- 光探针的光谱稳定性对于精确的多色光成像至关重要.
- 超分辨率显微镜中的高光强度挑战探测器的稳定性,影响数据的可重现性.
- 光漂白和光转换是当前光体的主要局限性.
研究的目的:
- 合成光谱稳定的胺.
- 评估它们对光漂白和光转换的光稳定性.
- 在活细胞超分辨率显微镜中展示它们的实用性.
主要方法:
- 新型罗达胺衍生物的顺序 Ru 和 Cu 催化合成.
- 化学分析以与现有的光体进行比较.
- 活细胞STED (刺激性排放耗尽) 纳米扫描以评估性能.
主要成果:
- 鉴定了对抗光转换的三甲的替代模式.
- 显示出优异的光稳定性和对光漂白和光转换的抗性.
- 在活细胞STED纳米镜中成功应用非蓝色标签.
结论:
- 开发了一种合成途径,使得高光稳定的胺.
- 这些新型探测器为苛刻的成像应用提供了更好的光谱稳定性.
- 非蓝色标签适用于高分辨率的活细胞成像.
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