需要糖溶性切换才能转化为内皮血统
Li Lai1, Erin Reineke2, Dale J Hamilton2
1Department of Cardiovascular Sciences, Center for Cardiovascular Regeneration (L.L., J.P.C.), Houston Methodist Research Institute, TX.
Circulation
|December 28, 2018
概括
天生的免疫信号触发了新陈代谢转向糖解,这对于纤维细胞转化为内皮细胞至关重要. 这一过程涉及表观遗传变化,并由ATP酸酶调节.
科学领域:
- 细胞生物学
- 代谢调节
- 表观遗传学
背景情况:
- 天生的免疫信号激活促进纤维细胞诱导内皮细胞转化.
- 代谢转变,特别是涉及糖溶解,涉及诱导多能干细胞的产生.
研究的目的:
- 研究新陈代谢变化的作用,特别是糖溶解,在先天免疫驱动的转化.
- 阐明细胞命运转换期间与先天免疫,新陈代谢和表观遗传修饰有关的分子机制.
主要方法:
- 使用海马和代谢流量测试来分析体外转化过程中的代谢变化.
- 使用Matrigel插头测试来评估糖解调节剂对转差的体内影响.
主要成果:
- 在先天性免疫激活后发生快速的糖溶性切换,先于内皮系标记的表达.
- 抑制糖解阻碍了诱导的内皮细胞生成,而其增强则改善了.
- 聚I:C刺激上调了与酸盐代谢相关的酶 (Slc25A1,ATP-酸盐酶),导致酸盐积累和酸盐乙化增加.
结论:
- 内在的免疫信号驱动一个对转差至关重要的糖解开关,由ATP酸酶调节.
- 这项研究揭示了代谢重编程与细胞转基因修饰之间的新联系.
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