相关实验视频
Updated: Jan 31, 2026

08:31
Antibody-Free Assay for RNA Methyltransferase Activity Analysis
Published on: July 9, 2019
7.6K
SETD3 是一种抗原基转移酶,可以预防原发性缩
Alex W Wilkinson1, Jonathan Diep2, Shaobo Dai3
1Department of Biology, Stanford University, Stanford, CA, USA.
Nature
|January 11, 2019
概括
科学家确定SET域蛋白3 (SETD3) 是负责在histidine 73中甲基化actin的酶. 这种动蛋白修饰对于小鼠的平滑肌肉功能和生育能力至关重要.
科学领域:
- 生物化学
- 分子生物学
- 细胞生物学
背景情况:
- 哺乳动物的actin在histidine 73 (His73) 的甲基化已被认可超过50年.
- 尽管它在物种中普遍存在和保存,但His73甲基化的功能和负责的酶仍然未知.
研究的目的:
- 找出催化了His73甲基化的酶.
- 阐明His73甲基化对行为动态和细胞过程的功能后果.
- 在体内调查SETD3和actin His73甲基化的生理作用.
主要方法:
- 蛋白质结构的确定 (X射线晶体) 以可视化SETD3-actin相互作用.
- 生物化学试验以评估His73甲基化对乙烯基核酸交换和丝组装的影响.
- 产生和分析SETD3缺乏的小鼠.
- 定量蛋白质组学用于识别SETD3基质.
- 在人子宫平滑肌细胞的体外研究.
主要成果:
- 鉴定出SET域蛋白3 (SETD3) 是actinHis73的特定甲基转移酶.
- 在SETD3的催化部位内,结构分析揭示了指向actinHis73的精确机制.
- 证明His73的甲基化减少了亚丁单体核酸的交换,并促进了丝的组装.
- 缺少SETD3的小鼠完全没有His73的甲基化和受损的生育能力 (孕产妇消极症).
- 证实SETD3是唯一负责生理性actin His73甲基化的酶.
- 人类子宫光滑肌细胞的信号诱导收缩受损.
结论:
- SETD3 是哺乳动物的胺甲基转移酶,负责actin His73的甲基化.
- 乙His73甲基化调节乙动力学,对平滑肌肉收缩性和女性生殖成功至关重要.
- 蛋白质胺甲基化是细胞功能的重要,但被低估的调节机制.
相关概念视频
Actin Treadmilling
9.7K
Actin filaments undergo polymerization and depolymerization from either end. The polymerization and depolymerization rates depend on the cytosolic concentration of free G-actins. The polymerization rate is generally higher at the plus or barbed end, while the depolymerization rate is higher at the minus or pointed end. At a steady state, critical concentration describes the concentration of free G-actin monomers at which the polymerization rate at the plus end is equal to that of the...
9.7K
Cancer Prevention
8.1K
Several factors can increase the risk of cancer in an individual. About 50% of cancer cases can be prevented by adopting a healthy lifestyle, regular exercise, eating healthy, and following a modest cancer prevention diet. Epidemiological studies have consistently shown that populations with vegetable and fruit-rich diets have reduced the incidence of cancer. On the other hand, populations who have a diet rich in animal fat, red meat, junk food, or high calories are predisposed to cancer.
Some...
Some...
8.1K
Introduction to Actin
6.6K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution. Actin coding genes are conserved within species and across...
6.6K
Actin Polymerization
8.6K
Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶ nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
8.6K
Prevention of Further Absorption of Poison
1.3K
In cases of acute poisoning, the primary objective is to prevent further absorption of the toxic substance into the body. Immediate interventions using various decontamination techniques targeting the gastrointestinal (GI) tract can achieve this. Decontamination is crucial to prevent poison from entering the systemic circulation, which involves washing affected areas with water and mild soap and removing contaminated clothing. Once external decontamination is done, attention must be turned to...
1.3K
Preventive Healthcare Services
2.2K
Preventive healthcare services keep people healthy via frequent check-ups, screening, and counseling. They primarily aid in disease prevention rather than treating an acute or chronic illness. Preventive treatment also keeps individuals productive and energetic, allowing them to work well into their retirement years. Examples of preventive care services include:
2.2K

