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在大脑皮层发育的轴突突出的亚细胞转录体和蛋白质体
Alexandros Poulopoulos1,2, Alexander J Murphy3, Abdulkadir Ozkan3
1Department of Stem Cell and Regenerative Biology, Center for Brain Science, and Harvard Stem Cell Institute, Harvard University, Cambridge, MA, USA. apoulopoulos@som.umaryland.edu.
Nature
|January 11, 2019
概括
研究人员开发了一种新的方法来绘制神经元生长中的分子, 这有助于理解神经回路的发展和潜在的再生.
科学领域:
- 神经科学
- 分子生物学
- 发育生物学
背景情况:
- 神经电路的发展需要精确的轴突连接,
- 增长包含用于投射特定增长和指导的分子机械.
研究的目的:
- 开发和应用一种新的方法来分析原生生长的分子组成.
- 识别增长内的分子专业化,有助于特定的神经电路的形成.
主要方法:
- 开发了生长分类和亚细胞RNA-蛋白质组映射.
- 应用该技术在小鼠大脑皮层中发展状突出.
- 从单个神经元亚型生成配对的亚细胞转录体和蛋白质体.
主要成果:
- 与母细胞体相比,在跨半球生长中确定了分子丰富.
- 在生长中发现增长调节激酶mTOR和相关mRNA的积累.
- 从单个体内隔离的生长中证明了配对RNA和蛋白质分析的可行性.
结论:
- 开发的方法提供了神经连接中的分子基质的系统级视图.
- 这些发现揭示了细胞下RNA与神经元发育中的蛋白质分布之间的关系.
- 这种方法可以促进电路形成,错误连接和神经再生的研究.
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