可逆促进体调解细菌相变,抗生素耐药性和肠道宿主适应性
Xiaofang Jiang1,2, A Brantley Hall2,3, Timothy D Arthur2
1Center for Microbiome Informatics and Therapeutics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
使用PhaseFinder算法探索了关键细菌适应的相变. 这揭示了宿主相关细菌中的众多DNA逆转区域 (逆转子),影响了抗生素耐药性和肠道殖民.
科学领域:
- 微生物学
- 基因组学
- 生物信息学
背景情况:
- 阶段变化使细菌能够适应不断变化的环境,这对感染和殖民至关重要.
- 不完全理解DNA逆转介导相变的多样性和功能意义.
研究的目的:
- 开发和应用一个算法 (PhaseFinder) 来量化DNA反转介导的相变.
- 在细菌基因组中系统地识别和表征可逆DNA区域 (反转子).
主要方法:
- 开发用于分析DNA反转的PhaseFinder算法.
- 在54,875个细菌基因组中进行系统的生物信息搜索.
- 对反转子位置,相关基因和对刺激的方向变化的分析.
主要成果:
- 在宿主相关细菌中发现了4686个基因间可逆DNA区域 (invertons).
- 调节细胞外产物的逆子与表面多样性和肠道殖民有关.
- 通过反转子观察到促进剂驱动的抗生素耐药性基因调节,抗生素治疗后的方向转移.
结论:
- 基因逆转介导的阶段变异很普遍,特别是在与宿主相互作用的细菌中.
- 在细菌适应过程中,包括管理抗生素耐药性的成本,inverton起着重要作用.
- 环境和宿主特异性因素导致逆转子的偏向分歧.
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