在性金属有机框架中封装的酶的增强活性
Weibin Liang1, Huoshu Xu2, Francesco Carraro
1Department of Chemistry and the Centre for Advanced Nanomaterials , The University of Adelaide , Adelaide , South Australia 5005 , Australia.
Journal of the American Chemical Society
|January 15, 2019
概括
在水友金属有机框架 (MOF) 中封装的酶在恶劣条件下保持活性. 然而,疏水性MOF无法保护酶,这凸显了MOF化学在生物仿真矿化应用中的重要性.
科学领域:
- 材料科学
- 生物化学
- 化学工程
背景情况:
- 金属有机框架 (MOF) 为封装生物大分子提供了一个有前途的平台.
- 使用MOF的仿生矿化可以在具有挑战性的环境中保护敏感的生物分子.
- MOF涂层的化学特性对于保持生物分子功能至关重要,但尚未得到充分研究.
研究的目的:
- 研究MOF涂层化学对封装酶的稳定性和活性的影响.
- 对抗各种恶劣条件的水友性和疏水性MOF的保护能力进行比较.
主要方法:
- 酶被封装在不同的MOF中 (MAF-7,ZIF-90,ZIF-8).
- 封装的酶受到高温,变性剂,蛋白质溶解剂和有机溶剂的影响.
- 在暴露于这些条件后测量了酶活性.
主要成果:
- 在水友性MOF (MAF-7,ZIF-90) 中封装的酶保持了显著的酶活性.
- 性MOF封装保护酶免受热,变质剂,蛋白酶和溶剂的影响.
- 在疏水性ZIF-8中封装的酶显示不活跃的催化酶和最小的尿酶保护.
结论:
- 在生物仿制矿物化过程中,MOF涂层的水友性对于维护酶功能至关重要.
- 疏水性MOF不适合在恶劣环境中保护酶.
- 在酶封装的稳定性和活性方面,MOF化学起着至关重要的作用.
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