一个Hendecad动机是偏好的异体螺旋-螺旋形成
Dale F Kreitler1, Zhihui Yao2, Jay D Steinkruger3
1Department of Chemistry , University of Wisconsin-Madison , Madison , Wisconsin 53706 , United States.
Journal of the American Chemical Society
|January 16, 2019
概括
由l-和d-多形成的异体螺旋螺旋,更喜欢11-残留的hendecad重复. 这一发现提升了对多相互作用和线圈设计的理解.
科学领域:
- 结构生物学
- 生物化学
- 蛋白质科学
背景情况:
- 在蛋白质结构中,卷-卷组件是常见的.
- 对于l-和d-之间的相互作用的了解很少.
- 之前的研究已经从M2-TM中确定了异体性线圈二元体.
研究的目的:
- 调查异体卷的序列重复偏好.
- 确定hendecad图案是否是对面螺旋配对的内在特征.
- 探索侧链修改对卷轴包装的影响.
主要方法:
- 三个M2-TM变体的结晶.
- 高分辨率的X射线晶体学
- 螺旋频率和卷轴半径的分析.
主要成果:
- 在所有三种变体中都观察到异体内线圈-线圈关联.
- 确认了对11-残留 (hendecad) 序列图案的偏好.
- 证明较小的侧链可以改善卷轴包装.
结论:
- 汉德卡德图案是异体螺旋形成的内在特征.
- 侧链工程可以优化卷轴包装.
- 提供了设计新型异体螺旋结构的基础.
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