内子是细胞对饥饿反应的调解者
Julie Parenteau1, Laurine Maignon1, Mélodie Berthoumieux2
1RNA Group, Département de Microbiologie et d'Infectiologie, Faculté de Médecine et des Sciences de la Santé, Université de Sherbrooke, Sherbrooke, Quebec, Canada.
Nature
|January 18, 2019
概括
在饥饿期间增强细胞存活率. 在营养物质枯竭的情况下, 移除内子会损害生长,
科学领域:
- 分子生物学
- 遗传学
- 细胞生物学
背景情况:
- 内子是基因内的非编码序列,在RNA拼接过程中被删除.
- 在真核细胞中产生功能性蛋白质时,剪接是必不可少的.
- 内子的进化持久性表明潜在的未被发现的功能.
研究的目的:
- 研究细胞适应饥饿的作用.
- 为了确定内部的物理存在是否会在营养物质枯竭的条件下影响细胞存活.
- 阐明引子可能赋予生存优势的分子机制.
主要方法:
- 系统删除已知酵母基因中的所有内基因.
- 在饥饿条件下对内部删除突变体的表型分析.
- 转录和遗传分析以确定涉及的调节途径.
主要成果:
- 在营养缺乏期间,缺乏内子的细胞的生长和存活显著受损.
- 观察到的内子对生存的影响与宿主基因表达水平无关.
- 通过TORC1和PKA通路增强了核糖体蛋白基因的抑制.
结论:
- 基因组中内子的物理存在在促进细胞在饥饿状态下存活起着至关重要的作用.
- 通过调节核糖体蛋白基因表达,引子有助于抗饥饿.
- 这些发现提供了关于内子和细胞适应机制的进化维护的见解.
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