和氧化对象结合在细胞染色体P450cam (CYP101A1) 中产生了新的合规状态
Alec H Follmer1, Sarvind Tripathi2, Thomas L Poulos1
1Departments of Molecular Biology and Biochemistry, Pharmaceutical Sciences, and Chemistry , University of California , Irvine , California 92697-3900 , United States.
Journal of the American Chemical Society
|January 24, 2019
概括
细胞染色体P450在开放和关闭状态之间发生变化. 新的结构显示,化物结合会诱导巨大的形状变化,开辟一个新的活性位点接入通道,这对P450酶催化至关重要.
科学领域:
- 生物化学
- 结构生物学
- 酵素学
背景情况:
- 细胞染色体P450 (P450s) 存在着不同的闭合和开放的构造状态.
- 与P450cam相似的氧对象结合,有利于开放状态,可能促进质子继电器和O-O键裂变.
- 氧-P450cam-Pdx中间体是不稳定的,但化物 (CN-) 复合物作为稳定的模仿物.
研究的目的:
- 为了阐明P450cam在与其氧化还原合作伙伴化物结合时的结构和构造变化.
- 研究P450催化机制中的形态动力学作用.
主要方法:
- P450cam-Pdx-CN-复合物的X射线结晶学
- 分析形状变化,包括残留物重新排列和通道形成.
主要成果:
- 确定了P450cam- Pdx复合体与CN- 的晶体结构.
- 化物结合引发了显著的形状变化,包括proline残留物的cis/trans异构化.
- 观察到大量的活性场所残留物重新排列,并形成新的活性场所接入道 (道2).
结论:
- P450cam-Pdx-CN-结构提供了关于P450形状灵活性的见解.
- 新发现的2通道支持先前关于基体结合和动态合的发现.
- 大型形状变化和动态是P450酶的催化功能的一部分.
更多相关视频
14:28Peptide-based Identification of Functional Motifs and their Binding Partners
Published on: June 30, 2013
13.0K
11:04Ion Mobility-Mass Spectrometry Techniques for Determining the Structure and Mechanisms of Metal Ion Recognition and Redox Activity of Metal Binding Oligopeptides
Published on: September 7, 2019
9.8K
相关概念视频
Ligand Binding and Linkage
5.6K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked. In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
5.6K
Ligand Binding and Linkage
4.1K
4.1K
Ligand Binding Sites
15.0K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
15.0K
Ligand Binding Sites
8.8K
8.8K
Conformity
48.2K
Conformity is the change in a person’s behavior to go along with the group, even if that person does not agree with the group.
48.2K
Balancing Redox Equations
62.0K
Electrochemistry is the science involved in the interconversion of electrical and chemical reactions. Such reactions are called reduction-oxidation, or redox reactions. These important reactions are defined by changes in oxidation states for one or more reactant elements and include a subset of reactions involving the transfer of electrons between reactant species. Electrochemistry as a field has evolved to yield sufficient insights on the fundamental principles of redox chemistry and multiple...
62.0K
