激素转移聚合物与实时监测
Luis A Navarro1, Alan E Enciso2, Krzysztof Matyjaszewski2
1Department of Mechanical Engineering and Materials Science , Duke University , 101 Science Drive , Durham , North Carolina 27708 , United States.
Journal of the American Chemical Society
|January 25, 2019
概括
葡萄糖氧化酶 (GOx) 能够在空气中进行表面激素聚合. GOx辅助原子转移基聚合 (ATRP) 改善了聚合物刷的生长动力学,并增强了抗污染特性,驱逐了更多的蛋白质.
科学领域:
- 聚合物化学
- 表面科学
- 生物材料工程
背景情况:
- 对于聚合物刷涂层来说,激光聚合非常重要,但对氧气非常敏感.
- 酶的氧化清除提供了可控聚合的潜在解决方案.
- 葡萄糖氧化酶 (GOx) 可以消耗氧气,在有氧条件下促进聚合.
研究的目的:
- 研究使用葡萄糖氧化酶 (GOx) 协助原子转移基聚合 (ATRP) 在环境空气中进行表面启动的聚合物刷生长.
- 评估GOx对聚合动力学和聚合物刷特性的影响.
- 评估GOx修饰的聚合物刷涂层的防性能.
主要方法:
- 在存在或缺少GOx的情况下,生物医学相关的聚合物 (POEGMA,PDMAEMA,PSBMA,PMSEA) 的表面启动ATRP.
- 使用石英晶微平衡 (QCM) 和局部表面等离子共振 (LSPR) 实时监测聚合动力学.
- 包括双分子终结在内的刷子生长的动态建模,以分析反应参数.
主要成果:
- 在GOx的帮助下,ATRP成功地从暴露在空气中的表面生产了聚合物刷.
- GOx显著增加了有效的动力链长度,繁殖率和聚合物刷生长的可重复性 (R2 > 0. 987).
- 与没有GOx的对照组相比,用GOx制备的聚合物涂层表现出优异的防性,排斥更多的人类血蛋白.
结论:
- GOx是一种有效且廉价的添加剂,可在露天条件下启动表面ATRP.
- 用GOx辅助的ATRP可以更好地控制聚合物刷的生长,并增强抗污染特性.
- 这种酶方法为开发具有更高性能的先进生物材料提供了有前途的策略.
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