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相关概念视频

RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
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相关实验视频

Updated: Jan 30, 2026

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
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Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

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拼接的密码深入

Suying Bao1, Daniel F Moakley1, Chaolin Zhang1

  • 1Department of Systems Biology, Department of Biochemistry and Molecular Biophysics, Center for Motor Neuron Biology and Disease, Columbia University, New York, NY 10032, USA.

Cell
|January 26, 2019
PubMed
概括
此摘要是机器生成的。

基因组序列背景显著影响RNA拼接,产生转录组多样性. 这些发现突显了DNA序列在调节基因表达和细胞功能的关键作用.

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科学领域:

  • 分子生物学
  • 基因组学
  • 转录组学

背景情况:

  • RNA拼接是一种关键的转录后修饰,产生转录组多样性.
  • 基因组序列背景对RNA拼接的影响一直是活跃的研究领域.

研究的目的:

  • 阐明基因组序列背景在RNA拼接中的作用.
  • 了解序列上下文如何对转录组多样性产生影响.

主要方法:

  • 基因组序列的分析及其与拼接模式的相关性.
  • 在不同基因组背景下进行比较研究.

主要成果:

  • 基因组序列背景是RNA拼接结果的关键决定因素.
  • 基因组中的特定序列元素会影响替代拼接事件.
  • 这种背景对于生成全谱转录组多样性至关重要.

结论:

  • 基因组序列上下文在RNA拼接中起着基本的作用.
  • 了解基于序列的拼接调节对于理解基因表达和细胞功能至关重要.