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一个用于产生室内特定心脏组织和疾病建模的平台
Yimu Zhao1, Naimeh Rafatian2, Nicole T Feric3
1Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, ON M5S 3E5, Canada.
Cell
|January 29, 2019
概括
研究人员开发了一种多功能平台,从多能干细胞设计出独特的人类心房和心室组织. 这项创新使得先进的药物测试和心脏病模型,
科学领域:
- 生物医学工程
- 干细胞生物学
- 心血管研究
背景情况:
- 人类多能干细胞衍生的心肌细胞为药物发现提供了潜力.
- 目前的限制包括心室规格和平台的多功能性.
- 克服这些挑战对于有效的心脏组织工程至关重要.
研究的目的:
- 开发一个可扩展的,细胞源无关的组织培养平台.
- 在电动节奏下对人工心脏组织进行药物测试.
- 设计出电生理学上不同的心房和心室组织.
主要方法:
- 使用一种新的塑料平台进行在线,非侵入性记录机械和电气性能.
- 组合定向电池差异化与电场调节.
- 工程化异极心脏组织和评估药物反应超过8个月.
主要成果:
- 成功设计出不同的心房和心室组织与室内特定的药物反应和基因表达.
- 在异极组织中表现出局限于空间的反应,
- 在8个月的电气调节后,使用患者衍生的细胞模拟多基性左心室缩.
结论:
- 开发的平台克服了心室规格和平台多功能性的局限性.
- 这项技术使得使用人类心脏组织进行强大的药物测试和疾病建模.
- 它代表着组织工程在再生医学和制药研究中的重大进步.
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