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高通量"FP-Tag"测试用于识别糖基转移酶抑制剂

Zhizeng Gao1, Olga G Ovchinnikova2, Bo-Shun Huang3

  • 1Department of Chemistry , University of British Columbia , Vancouver , British Columbia V6T 1Z1 , Canada.

Journal of the American Chemical Society
|January 31, 2019
PubMed
概括

研究人员开发了一种新的检测方法,以寻找细菌糖转移酶的抑制剂. 海洋天然产品被确定为有前途的化合物,针对这些关键的毒性因素.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...