灵长类视网膜中的和外围细胞的分子分类和比较分类学
Yi-Rong Peng1, Karthik Shekhar2, Wenjun Yan1
1Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Cell
|February 5, 2019
概括
研究人员使用单细胞RNA测序绘制了灵长类细胞. 这揭示了与外围视网膜和小鼠模型的关键差异,有助于理解人类视网膜疾病.
科学领域:
- 神经科学
- 基因组学
- 眼科 眼科
背景情况:
- 灵长类动物的高敏度视觉依赖于多个模型生物中缺少的视网膜区域.
- 由于这种可访问性差距,对体功能和疾病的理解是有限的.
研究的目的:
- 创建毛和外围视网膜的详细细胞地图.
- 将这些地图与小鼠视网膜细胞类型进行比较,并确定保存和分离的特征.
- 将细胞类型和基因表达与人类视网膜疾病联系起来.
主要方法:
- 在超过165,000个细胞上利用单细胞RNA测序 (scRNA-seq).
- 进行了毛,外围视网膜和小鼠视网膜的比较分析.
- 将与人类视网膜疾病相关的基因映射到特定细胞类型和视网膜区域.
主要成果:
- 鉴定了>60种细胞类型,其中>80%的细胞共享在细胞和周围细胞之间,但其比例和基因表达不同.
- 发现内部神经元类型的高保存,但灵长类动物和小鼠之间的投影神经元差异.
- 在人类中确立了关键的视网膜细胞类型的保存.
- 与特定细胞类型和视网膜区域相关的>190个疾病相关基因.
结论:
- 提供了灵长类人眼和视网膜的全面细胞图谱.
- 突出跨物种和视网膜区域的保存和分离的细胞程序.
- 在单细胞水平上研究人类视网膜疾病的框架.
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