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一个选择性的,基于蛋白质的光传感器,具有稀土元素的皮科莫尔亲和力

Joseph A Mattocks¹, Jackson V Ho¹, Joseph A Cotruvo¹

¹Department of Chemistry , The Pennsylvania State University , University Park , Pennsylvania 16802 , United States.

Journal of the American Chemical Society

|February 7, 2019

概括

一个新的光传感器LaMP1能够高灵敏地检测稀土元素. 这种工具揭示了细菌如何运输这些关键金属,

科学领域:

生物化学
环境科学
微生物学

背景情况:

稀土元素的检测对于采矿和回收至关重要.
现有的化物 (Ln) 检测方法缺乏灵敏度和速度.
了解Ln的生物作用需要敏感的检测工具.

研究的目的:

开发一种基因编码的光传感器,用于选择性Ln检测.
在Methylobacterium extorquens中研究Lns的细胞内运输.
在细菌中探索潜在的Ln吸收系统.

主要方法:

基于兰莫杜林的光传感器 (LaMP1) 的设计.
描述LaMP1对各种金属离子的反应.
使用LaMP1研究Methylobacterium extorquens中的Ln传输.

主要成果:

对于所有Ln (III) 离子,LaMP1具有很高的选择性和敏感性 (明显的Kds为10-50 pM).
已证明早期Lns (La(III) -Nd(III)) 的选择性传输到M. extorquens的细胞质中.
提供了一种涉及分泌化剂的新型Ln(III) 吸收系统的证据.

结论:

对于敏感和选择性的REE检测,LaMP1是一个强大的工具.
发现了一种令人惊的细菌细胞内Ln传输机制.
在环境和工业应用中,LaMP1可以推进REE分析.

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