一种N-化金属酶构建了链杆菌素的药
Tai L Ng1, Roman Rohac2, Andrew J Mitchell3
1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Nature
|February 8, 2019
概括
科学家们发现了SznF, 这是一种在致癌物和癌症药物中产生N-酸酶的酶. 这种金属酶
科学领域:
- 生物化学
- 酵素学
- 有机化学
背景情况:
- 酸化合物是显著的致癌物和化疗药物.
- 负责N- 素组生物合成的酶仍未确定.
研究的目的:
- 识别和描述参与N-nitroso化合物生物合成的酶.
- 通过SznF阐明N-基组形成的机制.
主要方法:
- 用于研究酶活性的生物化学测试.
- 使用X射线结晶学的SznF结构特征.
- 针对位点的突变发生,以调查活性位点的功能.
主要成果:
- 一种金属酶SznF催化了N-甲基-L-氨酸产物的形成.
- SznF具有两个不同的活性位点,用于不同的铁共因子的催化.
- 该酶促进N-N键的形成,扩大非海姆铁酶的已知功能.
结论:
- SznF是第一个专门用于N-nitroso组组合的酶.
- 这一发现揭示了一种新的生物合成途径,有助于了解细菌中的N-代谢物多样性.
- 这项工作扩大了N-N键形成中的金属酶的催化范围.
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