相关实验视频
Updated: Jan 29, 2026

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Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
12.8K
癌症中的循环RNA景观
Josh N Vo1, Marcin Cieslik1, Yajia Zhang1
1Department of Computational Medicine and Bioinformatics, University of Michigan, Ann Arbor, MI 48109, USA; Michigan Center for Translational Pathology, University of Michigan, Ann Arbor, MI 48109, USA.
Cell
|February 9, 2019
概括
我们开发了一种新的RNA测序方法, 在2000多个癌症样本中发现循环RNA. 这种方法确定了新型circRNAs作为前列腺癌和尿液样本的潜在生物标志物.
科学领域:
- 分子生物学
- 基因组学
- 癌症研究
背景情况:
- 循环RNAs是稳定的RNA分子,在基因调节中具有独特的结构和作用.
- 在癌症中检测和表征circRNA对于了解疾病机制和开发诊断至关重要.
- 现有的circRNA丰富和检测方法存在局限性.
研究的目的:
- 开发和验证用于癌症综合性circRNA检测的增强RNA测序协议.
- 建立从瘤组织中获取的最广泛的circRNAs目录,
- 为癌症诊断确定circRNA生物标志物,包括在尿液中检测.
主要方法:
- 在2000多个癌症样本中采用了exome捕获RNA测序协议.
- 捕获测序与Ribo-Zero和RNase R方法进行circRNA丰富的比较.
- 开发一个MiOncoCirc数据库来对检测到的circRNA进行分类.
主要成果:
- 与其他方法相比,捕获序列显著改善了circRNA的丰富性,并保持了精确的圆向线性比率.
- 在MiOncoCirc数据库中提供了直接在瘤组织中检测到的最全面的circRNAs目录.
- 在尿样中检测出前列腺癌的潜在生物标志物.
- 发现了一种新型的循环转录:由不同基因的前体形成的阅读循环RNA.
结论:
- 外体捕获RNA测序协议是癌症中circRNA检测和表征的优越方法.
- MiOncoCirc是癌症研究和基于circRNA的诊断和治疗方法的开发的宝贵资源.
- 在尿液中检测到的circRNAs显示为前列腺癌检测的非侵入性生物标志物.
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