通过电阻脉冲纳米孔传感观察到带有酸盐覆盖的金纳米集团的带诱导的结构变化
Bobby D Cox1, Patrick H Woodworth1, Peter D Wilkerson1
1Department of Physics , Virginia Commonwealth University , Richmond , Virginia 23284 , United States.
Journal of the American Chemical Society
|February 19, 2019
概括
这项研究使用α-hemolysin (αHL) 纳米孔检测黄金纳米集群. 电信号的变化与连接体质量相关,使表面修饰的特征成为可能.
科学领域:
- 生物物理
- 纳米技术
- 分析化学
背景情况:
- 纳米孔传感传统上分析生物聚合物.
- 新兴应用包括水溶性金属集群的特征.
- 阿尔法-血解素 (αHL) 是一种生物纳米孔.
研究的目的:
- 调查αHL纳米孔的使用,以检测酸盐覆盖的金纳米集群.
- 分析不同封闭配体对纳米孔信号的影响.
- 探索纳米孔探测以表征粒子内部表面的变化.
主要方法:
- 使用αHL纳米孔进行电阻脉冲传感.
- 用p-mercaptobenzoic acid (p-MBA),tiopronin (TP) 和化PEG7 (S-PEG7) 覆盖的黄金纳米团的检测.
- 对被困在孔中的单个纳米集群的电流波动和子状态的分析.
主要成果:
- 个别的黄金纳米集群在αHL孔内表现出明显的电流波动.
- 观察到的电流步骤与封闭配体 (p-MBA,TP,S-PEG7) 的质量相关.
- 发现连接体质量是纳米孔信号特征的缩放因素.
结论:
- 使用αHL的纳米孔传感可以根据它们的表面配体区分金色纳米集群.
- 该技术显示了金属纳米集群的粒子内部表面修饰的潜力.
- 这种方法为分析纳米粒子表面化学提供了一种新方法.
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