蝙蝠流感病毒通过MHCII类蛋白质跨物种进入
Umut Karakus1, Thiprampai Thamamongood2,3,4,5, Kevin Ciminski2,3
1Institute of Medical Virology, University of Zurich, Zurich, Switzerland.
Nature
|February 22, 2019
概括
蝙蝠流感A病毒是一种潜在的流行性威胁,利用主要细胞相容性复合体II类 (MHC-II) 进入细胞. 这一发现揭示了广泛的宿主范围,影响了动物传染病监测和控制策略.
科学领域:
- 病毒学
- 免疫学
- 遗传学
背景情况:
- 主要来自鸟类的动物性流感A病毒构成流行病风险.
- 在蝙蝠中发现了新型流感A型病毒,
- 蝙蝠流感A病毒的异常受体结合特性仍然没有特征.
研究的目的:
- 确定蝙蝠流感A病毒的进入机制和宿主热带.
- 为了确定蝙蝠流感A病毒感染的宿主因素.
- 评估蝙蝠流感A病毒的动物感染潜力.
主要方法:
- 敏感细胞和不敏感细胞的转录形状.
- 全基因组CRISPR-Cas9查以确定宿主决定因素.
- 用蝙蝠流感A病毒感染小鼠的实验.
主要成果:
- 主体组织相容性复合物II类 (MHC-II),特别是人类白细胞抗原DR (HLA-DR),对于蝙蝠流感A病毒的进入至关重要.
- 对HLA-DR表达的基因修饰改变了细胞对感染的敏感性.
- 不同物种的MHC-II表达赋予了敏感性,表明了广泛的热带性.
- 缺乏MHC- II的小鼠对蝙蝠流感A病毒感染有抗性.
结论:
- 作为蝙蝠流感A病毒的关键入口媒介.
- 蝙蝠流感A病毒具有由MHC-II介导的广泛的脊椎动物热带性.
- 这一发现对了解和控制蝙蝠传播的动物流感有重大影响.
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