用于组织工程的刺激响应,五,纳米纤维水凝
Journal of the American Chemical Society
|March 5, 2019
概括
我们开发了新的基凝, 这些可注射材料支持细胞生长,并为神经组织工程和移植疗法提供潜力.
科学领域:
- 生物材料科学
- 纳米技术
- 组织工程
背景情况:
- 短是多功能构建块,可自组装成功能性水凝生物材料.
- 超分子组合为神经组织工程应用提供了有吸引力的方法.
研究的目的:
- 报告一个新的类型的短,五残留, 形成水凝与纳米纤维结构.
- 描述这些五水凝的机械性能和自组装行为.
- 评估这些水凝的细胞兼容性和保护能力,用于细胞输送和神经组织工程.
主要方法:
- 使用风学和光谱分析机械性质以及序列变化,pH值和度的影响.
- 传输电子显微镜 (TEM) 和冷电子显微镜 (Cryo-EM) 可视化了纤维的形态.
- 分子动力学模拟证实了实验中的凝行为.
- 细胞封装和增殖试验使用了寡细胞原生细胞 (OPC).
主要成果:
- 七个未经修改的在低度 (<3重%) 形成了强大的水凝,具有剪切稀释和快速自我愈合的特性.
- 体自组成扭曲的带状纤维 (直径几十纳米) 与粉样周期性.
- 这些水凝支持OPC封装,扩散和3D过程扩展.
- 这些水凝保护了OPC在注射过程中的机械应力,证明了它们作为细胞载体的潜力.
结论:
- 开发的可注射快速组装五 (RAPID) 水凝具有可调的机械和结构性质.
- 这些水凝因其可注射性,细胞相容性和保护能力而适用于细胞输送和神经组织工程.
- 这些短自组合成功能纳米纤维水凝为再生医学开辟了新的途径.
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