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相关概念视频

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons09:21

Phenotypic Profiling of Human Stem Cell-Derived Midbrain Dopaminergic Neurons

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This protocol describes the cell culturing of human midbrain dopaminergic neurons, followed by immunological staining and the generation of neuronal phenotypic profiles from acquired microscopic high-content images allowing the identification of phenotypic variations due to genetic or chemical...
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Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression10:54

Reliable Identification of Living Dopaminergic Neurons in Midbrain Cultures Using RNA Sequencing and TH-promoter-driven eGFP Expression

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In Parkinson's Disease (PD), Substantia Nigra (SNc) dopaminergic neurons degenerate, leading to motor dysfunction. Here we report a protocol for culturing ventral midbrain neurons from a mouse expressing eGFP driven by a Tyrosine Hydroxylase (TH) promoter sequence, harvesting individual fluorescent neurons from the cultures, and measuring their transcriptome using...
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Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains08:45

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

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The causes of degeneration of midbrain dopaminergic neurons during Parkinson’s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of...
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Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice09:35

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice

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This protocol describes two different environmental manipulations and a concurrent brain infusion protocol to study environmentally-induced brain changes underlying adaptive behavior and brain repair in adult...
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Primary Culture of Mouse Dopaminergic Neurons11:58

Primary Culture of Mouse Dopaminergic Neurons

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various...
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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area09:54

Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area

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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in...
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相关实验视频

Updated: Jan 8, 2026

Environmental Modulations of the Number of Midbrain Dopamine Neurons in Adult Mice
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心脏衰竭中的线粒体功能障碍

Anupam A Kumar1, Daniel P Kelly1, Julio A Chirinos1,2

  • 1From the University of Pennsylvania Perelman School of Medicine, Philadelphia (A.K., D.P.K., J.C.).

Circulation
|March 12, 2019
PubMed
概括

线粒体功能障碍有助于心力衰竭的运动不耐受性 (HFpEF). 针对线粒体可以改善症状,并为HFpEF患者提供有前途的治疗途径.

科学领域:

  • 心脏病学
  • 线粒体生物学
  • 运动生理学

背景情况:

  • 心脏衰竭与保存的喷射分数 (HFpEF) 是一个异构的综合征.
  • 运动不耐受是HFpEF的主要症状,源于心脏和外围因素.
  • 在HFpEF中,线粒体功能障碍与氧气利用障碍和运动不耐受有关.

研究的目的:

  • 审查线粒体生物学在HFpEF病理生理学的作用.
  • 讨论评估人类线粒体功能的方法.
  • 探索线粒体作为HFpEF的治疗点.

主要方法:

  • 在HFpEF中对线粒体功能的文献综述.
  • 对评估人类线粒体功能的现有方法的分析.
  • 检查线粒体功能障碍与HFpEF相关的证据.

主要成果:

  • 线粒体异常在HFpEF中显著影响氧气利用和运动不耐受.
  • 在临床环境中存在各种方法来评估线粒体功能.
  • 线粒体功能障碍是HFpEF病理生理学的关键因素.

结论:

关键词:
核磁共振运动不耐受心脏衰竭与保存的喷射分数线粒体功能

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  • 线粒体功能是HFpEF的一个关键治疗点.
  • 针对线粒体功能的临床试验正在进行中,对开发新疗法至关重要.
  • 对线粒体剂的进一步研究有望改善HFpEF症状.