Apd1 和 Aim32 是比西斯蒂迪尼尔协调非风险蛋白的原型
Kathrin Stegmaier, Catharina M Blinn, Dominique F Bechtel
1Max-Planck-Institut für Chemische Energiekonversion , Stiftstrasse 34-36 , D45470 Mülheim an der Ruhr , Germany.
Journal of the American Chemical Society
|March 19, 2019
概括
这项研究揭示了酵母蛋白Apd1和Aim32中的特定的氨酸和氨酸残留物对于细胞在氧化还原应激下生存至关重要. 这些蛋白质含有独特的 [2Fe-2S] 集群,显示铁硫集群蛋白质的融合进化.
科学领域:
- 生物化学
- 分子生物学
- 生物有机化学
背景情况:
- Apd1 (细胞质酵母) 和Aim32 (线粒体酵母) 具有类似于硫素的铁素 (TLF) 域.
- 这些蛋白质在细菌,植物,真菌和病原性真核生物中保存,但在甲基动物中不存在.
研究的目的:
- 调查Apd1和Aim32TLF域内的重要残留物.
- 描述 [2Fe-2S] 星团及其在Apd1中的协调.
- 探索 [2Fe-2S] 集群含有的蛋白质的进化趋同.
主要方法:
- 化学遗传实验以评估细胞在氧化还原应激下的生存能力.
- 紫外线,EPR和莫斯巴尔光谱分析 [2Fe-2S] 星团.
- 定位突变 (His到Cys变体) 来探测配体的作用.
主要成果:
- 在C-X8-C-X24-75-H-X-G-G-H基因中保存的氨酸和氨酸残留物对于酵母在氧化还原应激下生存至关重要.
- 光谱分析确定了Cys207,Cys216,His255和His259为Apd1中的 [2Fe-2S]星团的配体.
- 与二氧化酶和里斯克集群相比,Apd1 的 [2Fe-2S] 集群具有不同的氧化还原潜力和 pK 值.
- 工程 Apd1 变体在 [2Fe-2S] 集群协调 (bishistidinyl,单基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基基
结论:
- 该研究将Apd1和Aim32蛋白质定义为酵母体中氧化还原平衡的重要组成部分.
- Apd1拥有一个前所未有的 [2Fe-2S] 集群,具有灵活的协调,突出显示了趋同的进化.
- 这些发现提供了铁硫蛋白的多样性作用和进化途径的见解.
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