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通过自我报告的光敏化剂在现场监测亡过程

Tianfu Zhang¹, Yuanyuan Li¹, Zheng Zheng¹

¹Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Institute of Molecular Functional Materials, Division of Life Science and State Key Laboratory of Molecular Neuroscience, Department of Chemical and Biological Engineering , The Hong Kong University of Science and Technology , Clear Water Bay, Kowloon , Hong Kong , China.

Journal of the American Chemical Society

|March 29, 2019

概括

一种新的自报光敏剂 (PS) 通过追踪其光迁移,可实时监测光动力疗法 (PDT). 这允许精确的剂量控制,最大限度地减少副作用并改善治疗结果.

科学领域:

生物医学工程
摄影化学
分子成像

背景情况:

光动力疗法是一种有前途的癌症治疗方法.
过量服用PDT可能会导致由于强烈的光敏剂 (PSs) 和高光强度而导致过度的光毒性.
目前用于监测治疗反应的方法往往复杂,现场进行,并且延迟.

研究的目的:

开发一种自我报告的阴离子光敏感剂 (PS),用于现场实时监测PDT.
在PDT诱导的亡过程中研究PS的转位机制.
在PDT中展示精确剂量控制和减少副作用的潜力.

主要方法:

合成一种具有聚合诱导排放特性的新型阴阳性PS,TPE-4EP+.
使用光显微镜在PDT期间实时跟踪TPE-4EP+从线粒体到细胞核的转移.
使用分子电荷工程来阐明正电荷在DNA相互作用和转移中的作用.

主要成果:

TPE-4EP+具有聚合诱导的排放,使其具有自我报告能力.
在PDT诱导的亡过程中,PS成功地经历了线粒体到核的转移,通过光迁移可视化.
分子电荷工程证实,由于多价值正电荷,与DNA的增强相互作用驱动了现场转移.

结论:

开发的TPE-4EP+可通过光迁移实时地对PDT进行实地监测.
这种自我报告的PS有助于精确的剂量控制,减轻过度的光毒性和潜在的副作用.
这种方法为下一代光敏剂铺平了道路, 具有完善的PDT综合诊断功能.

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