病原性大肠杆菌利用注射组分从受感染的宿主细胞中提取营养
Ritesh Ranjan Pal1, Amit K Baidya1, Gideon Mamou1
1Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, POB 12272, The Hebrew University of Jerusalem, 91120 Jerusalem, Israel.
Cell
|April 2, 2019
概括
像EPEC这样的病原细菌直接从宿主细胞中提取营养, 这种宿主营养提取 (HNE) 机制允许病原体在营养缺乏的环境中壮成长.
科学领域:
- 微生物学
- 细胞生物学
- 病原体与宿主之间的相互作用
背景情况:
- 肠道微生物群和肠道上皮通过快速的营养消耗来限制病原体的生长.
- 病原体必须制定策略来克服宿主殖民的营养竞争.
研究的目的:
- 研究病原体,特别是肠病原性大肠杆菌 (EPEC) 如何规避营养限制以殖民宿主.
- 确定使病原体从宿主细胞中获取营养的分子机制.
主要方法:
- 使用EPEC作为一种模型病原体来研究宿主细胞的营养获取.
- 鉴定和描述了参与宿主营养提取 (HNE) 的CORE蛋白质复合体.
- 研究了不同细菌结构中的CORE复合体的进化关系和功能保存.
主要成果:
- EPEC采用一种称为宿主营养提取 (HNE) 的过程,从受感染的宿主细胞中获取营养.
- 一种新的内膜蛋白质复合体CORE被确定为HNE必需且足够的.
- CORE促进了纳米管状结构的形成,以直接与宿主细菌接触,与注射体不同.
- 包括鞭毛在内的各种细菌来源的保存的CORE复合物可以恢复EPEC中的HNE功能.
结论:
- 主体营养提取 (HNE) 是病原体殖民营养有限的的关键毒性策略.
- CORE复合物及其相关的纳米管结构代表了病原体中营养获取的保存机制.
- 了解HNE提供了细菌病变和潜在治疗点的见解.
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