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在Drosophila胚胎中的Antennapedia蛋白表达的定位和调节

S B Carroll, R A Laymon, M A McCutcheon

|October 10, 1986

概括

在Drosophila中,Antennapedia (Antp) 基因指导胸部部段的发育. 超比索拉克斯 (Ubx) 蛋白可能直接抑制Antp基因表达,揭示了一个关键的调节相互作用.

科学领域:

发育生物学发展生物学
遗传学是一个遗传学.
分子生物学分子生物学

背景情况:

同源的Antennapedia (Antp) 基因对Drosophila胸部段区分至关重要.
了解Antp基因调节是解读发育途径的关键.

研究的目的:

在Drosophila胚胎中定位Antp蛋白表达.
研究其他遗传因素对Antp基因表达的调节.

主要方法:

针对重组Antp蛋白的抗体被用于整体安装胚胎局部化.
在突变胚胎中分析Antp表达.
对Antp和Ultrabithorax (Ubx) 蛋白质进行双重标记.

主要成果:

Antp蛋白位于胸部胚胎表皮和神经系统部分的核中.
Antp的表达受到 bithorax 综合体,同源位置和 Antp 本身的调节.
Ubx蛋白分布表明它可能直接抑制Antp基因表达.

结论:

Antp蛋白在胸部部位的发育中起着至关重要的作用.
多种遗传因素调节Antp表达,Ubx被确定为潜在的直接负调节者.

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