细胞透多硫化物增长和降解的单分子动力学
Gökçe Su Pulcu1, Nicole Stéphanie Galenkamp2, Yujia Qing1
1Department of Chemistry , University of Oxford , Oxford OX1 3TA , United Kingdom.
Journal of the American Chemical Society
|April 2, 2019
概括
可生物降解的细胞透多硫化物 (CPD) 可提供治疗效果. 它们的长度由合成和降解动力学控制,决定了细胞向,自循环是主要的分解途径.
科学领域:
- 聚合物化学
- 生物材料科学
- 纳米技术
背景情况:
- 将治疗剂输入目标细胞是复杂的.
- 可生物降解的细胞透多硫化物 (CPD) 提供了细胞透和细胞内向的解决方案.
- 聚合物长度极大地影响细胞局部化和向传递效率.
研究的目的:
- 研究CPD聚合物生长和降解的动力学.
- 在特定的合成条件下预测可实现的链条长度.
- 阐明细胞内脱聚合的主要途径.
主要方法:
- 使用单分子纳米反应器监测CPD动力学.
- 测量了聚合物生长和降解速度.
- 合成和脱聚变的确定速率常数.
主要成果:
- 含有40个单位的CPD在200毫米滴醇度下迅速合成 (pH7.4时1秒,pH8.4时0.3秒).
- 速率常数可以预测在不同条件下可实现的链条长度.
- 细胞内降解主要是通过自循环发生,而不是由硫醇的链内裂解.
结论:
- 通过合成条件,可以精确控制CPD的长度.
- 了解降解动力学对于优化向药物输送至关重要.
- 自循环是细胞内CPD脱聚合的主要机制.
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