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使用基于的磁共振探测器选择性结合和量化
Kang Du1, Agnes E Thorarinsdottir1, T David Harris1
1Department of Chemistry , Northwestern University , Evanston , Illinois 60208-3113 , United States.
Journal of the American Chemical Society
|April 5, 2019
概括
一种基于的新型磁性化学交换和转移 (PARACEST) 探测器可以选择量化 (Ca2+) 离子. 这种PARACEST探测器为Ca2+提供了独立于度的测量,从而推进了磁共振传感.
科学领域:
- 无机化学
- 磁共振成像 (MRI)
- 生物医学传感
背景情况:
- 准确量化生物相关的子如Ca2+对于理解生理过程至关重要.
- 现有的磁共振 (MR) 检测阴离子方法在生理条件下经常面临选择性和量化方面的挑战.
- 超磁性化学交换和转移 (PARACEST) 探测器为敏感和选择性的分子成像提供了潜力.
研究的目的:
- 开发和描述一种基于的新型PARACEST探测器,用于选择性Ca2+检测和量化.
- 在生理条件下评估探测器的性能,评估对干扰离子的选择性.
- 使用PARACEST建立一个独立于度的Ca2+测量的比度方法.
主要方法:
- 基于的PARACEST探测器 (LCo) 的合成和表征,其中包括CEST活性组和皇冠以太.
- 使用单晶X射线衍射,固态磁性测量和H NMR光谱,对阴离子结合 (Na++,Mg++,K++,Ca+) 的研究.
- 在生理条件下基于溶液的PARACEST测量,以确定结合亲和性,选择性,并开发一个比度量化方法.
主要成果:
- 该LCo复合物选择性地将Ca2+和Na+结合在其皇冠以太部分中,表现出特异性协调和磁性异构性.
- 观察到69 ppm ([LCoNa]+) 和80 ppm ([LCoCa]2+) 的明显的CEST峰值,表明可逆结合和高的Ca2+选择性.
- 通过对CEST峰值强度 (CEST80 ext{ ppm}/CEST69 ext{ ppm}) 的比度分析,可以独立于探针度成功量化Ca2+.
结论:
- 过渡金属 PARACEST 探针可以提供离子度的度独立测量,以 Ca2+ 为例.
- 这项研究首次使用PARACET对Ca2+进行了量化测量,克服了先前方法的局限性.
- 开发的探测器和方法为设计生物系统中的先进MRI探测器提供了有前途的新途径.
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