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微生物纳米线的结构揭示了通过微米传输电子的堆叠体
Fengbin Wang1, Yangqi Gu2, J Patrick O'Brien3
1Department of Biochemistry and Molecular Genetics, University of Virginia School of Medicine, Charlottesville, VA 22908, USA.
Cell
|April 6, 2019
概括
微生物纳米线对于电子运输至关重要,但令人惊的是,它们是由OmcS蛋白组成的,而不是Pila. 这种OmcS结构解释了它们在细菌呼吸中的高导电性和作用.
科学领域:
- 微生物学
- 生物物理
- 结构生物学
背景情况:
- 在长距离电子传输 (>10μm) 过程中,Geobacter sulfurreducens使用称为微生物纳米线的蛋白质丝.
- 这些纳米线之前被确定为由PilA蛋白组成的IV型Pili.
研究的目的:
- 确定微生物纳米线组装和功能在Geobacter中的结构基础.
- 阐明这些电子传输网络的高导电性.
主要方法:
- 使用冷电子显微镜确定高分辨率 (3.7 Å) 的Geobacter sulfurreducens纳米线.
- 在野生型和突变菌株 (ΔomcS) 上进行了比较导电性测量.
主要成果:
- 这项研究表明,Geobacter sulfurreducens纳米线主要由六合体细胞染色体OmcS组成,而不是Pila.
- 该结构显示了OmcS的微米长的聚合,其边缘密切包装 (∼3.5-6 Å) 和独特的跨子单元接口.
- 与 ΔomcS 菌株的纤维相比,野生类型的 OmcS 纤维具有 100 倍的导电性.
结论:
- OmcS是Geobacter sulfurreducens微生物纳米线的关键结构和导电组成部分.
- 在OmcS纤维中独特的血包装和协调解释了它们显著的电子传输能力.
- 这一发现为土壤细菌如何通过电子转移进行细胞外呼吸和能量共享提供了洞察力.
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