从药物集成的反意义核酸组装的二合一化基因来逆转化学阻力
Quanbing Mou1, Yuan Ma1, Fei Ding1
1School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites , Shanghai Jiao Tong University , 800 Dongchuan Road , Shanghai 200240 , China.
Journal of the American Chemical Society
|April 10, 2019
概括
研究人员开发了一种新的"化学物质"输送系统, 结合化疗和基因疗法, 这种系统将抗癌药物直接集成到DNA中, 提高治疗效率并恢复药物敏感性.
科学领域:
- 生物医学工程
- 纳米技术
- 癌症治疗
背景情况:
- 抗药性癌症是一个重大的临床挑战.
- 结合化疗和基因疗法为克服抗药性提供了一个有前途的策略.
- 现有的药物输送系统难以有效地同时输送化疗剂和核酸,因为它们的特性不同.
研究的目的:
- 开发一种新的纳米药物输送系统 (DDS),将化疗和基因疗法整合到抗药性癌症中.
- 通过将抗癌药物纳入具有反意义序列的DNA来创建"化学物质".
- 为了证明这个集成的DDS的协同抗瘤作用.
主要方法:
- 通过将核类型的floxuridine (F) 纳入反意义DNA序列,取代胺 (T) 来合成化学物质.
- 将化基因与聚烯酸结合在一起,形成球形核酸 (SNA) 样的纳米结构.
- 在小鼠模型中评估了DDS的细胞进入,基因下调,药物释放和体内抗瘤功效.
主要成果:
- 化基DDS在没有转染剂的情况下迅速进入细胞.
- 观察到药物耐药性基因的有效下调.
- 实现了持续释放化疗药物,导致皮下和正位肝癌模型中的瘤显著减少.
结论:
- 新型化学DDS有效地结合化疗和基因疗法治疗耐药癌症.
- 这种综合方法克服了同时使用不同治疗剂的局限性.
- 化学生成策略具有提高癌症治疗结果的巨大潜力.
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