细菌中链3-基脂肪酸代谢物激发Arabidopsis植物的免疫力
Alexander Kutschera1, Corinna Dawid2, Nicolas Gisch3
1Chair of Phytopathology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising-Weihenstephan, Germany.
植物利用LORE受体检测中链3-基脂肪酸 (mc-3-OH-FA),而不是脂多糖,作为关键的细菌免疫触发剂. 特定的mc-3-OH-FA,如 (R) -3-基酸,激活植物免疫力.
科学领域:
- 植物免疫力
- 微生物生物化学
- 分子生物学
背景情况:
- 植物细胞表面受体检测分子非自我签名以启动免疫反应.
- 来自格拉姆阴性细菌脂多糖的脂质A是一种已知的非自我签名.
- 受体激酶LIPOOLIGOSACCHARIDE-SPECIFIC REDUCED ELICITATION (LORE) 调解了植物对某些细菌的免疫力.
研究的目的:
- 调查由LORE受体识别的特定分子触发器.
- 确定中链3-基脂肪酸 (mc-3-OH-FA) 是否引起了依赖于LORE的免疫.
- 阐明在引发植物免疫反应时对mc-3-OH-FA的结构要求.
主要方法:
- 中链3-基脂肪酸 (mc-3-OH-FA) 的合成
- 在植物中使用合成和细菌脂多糖净化的mc-3-OH-FA.
- 使用各种细菌化合物 (脂质A,脂多糖,脂,脂,乙烯基同素-乳酸盐) 评估依赖于LORE的免疫反应.
主要成果:
- 合成和细菌的mc-3-OH-FA引起了植物对LORE的依赖性免疫力.
- 免疫反应是特定于mc-3-OH-FA链长和化,其中 (R) - 3 - 基酸是最强的诱导因子.
- 含有mc-3-OH-acyl组但缺乏自由mc-3-OH-FA的细菌化合物,如脂质A和脂多糖,不会触发LORE依赖的反应.
结论:
- 植物感知到简单的细菌代谢物,特别是中链3脂肪酸,通过LORE受体激活免疫反应.
- LORE受体的识别与其在感知脂质糖类中的作用不同.
- 这一发现揭示了基于检测低复杂度细菌代谢物的植物微生物相互作用的新机制.
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