硫化碳化物合成用于电压成像
Journal of the American Chemical Society
|April 13, 2019
概括
我们开发了新的电压敏感的光染料, 这些染料克服了以前的局限性,提供了更好的电压敏感性和超出典型光素波长的混合化学遗传电压成像应用.
科学领域:
- 有机化学
- 生物物理化学
- 分子成像
背景情况:
- 碳化物具有红移光谱,但患有高pKa和循环问题.
- 现有的电压敏感探测器通常在光谱范围或稳定性方面存在局限性.
- 素衍生物广泛使用,但其激发/发射概况仅限于较短的波长.
研究的目的:
- 设计和合成基于改造的碳化物支架的新型电压敏感光指示器.
- 克服碳化素的局限性,特别是高pKa和循环.
- 开发适用于波长超出典型光素范围的电压成像的探测器.
主要方法:
- 通过化和硫化对碳化物进行修改.
- 开发一种化硫化碳化合物的合成途径.
- 在烯烯分子电线支架中加入改性染料,以产生对碳电压敏感的光体 (碳VF染料).
- 染料特性包括pKa,光谱形状和电压敏感性.
- 使用酶激活的原染料进行化标签策略的演示.
主要成果:
- 化降低了碳素的pKa值至5.2,而硫化防止了循环.
- 新的 carboVF 染料,特别是 carboVF2.1 ((OMe).Cl,具有 >560 nm 的激发/发射.
- 实现了高电压灵敏度 (> 30% ΔF/F每 100 mV).
- 这些染料与其他光体相容,如绿色光蛋白.
- 在实验室和活细胞/神经元中,一种亲染色策略使酶激活的光恢复成为可能.
结论:
- 化硫碳素是一种有前途的新型电压敏感染料.
- 这些染料能够在更长的波长下进行电压成像, 克服现有探测器的局限性.
- 开发的探针适用于混合化学遗传电压成像应用.
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