对压力敏感和奥斯莫利特调节的眼镜 γ-晶体的液相分离
Süleyman Cinar1, Hasan Cinar1, Hue Sun Chan2
1Physical Chemistry I - Biophysical Chemistry, Faculty of Chemistry and Chemical Biology , TU Dortmund , Otto-Hahn-Strasse 4a , 44227 Dortmund , Germany.
Journal of the American Chemical Society
|April 16, 2019
概括
由液相分离 (LLPS) 驱动的生物分子凝聚物对压力有着惊人的敏感性. 深海生物可能使用三甲基胺-N-氧化物来稳定这些必需的蛋白质滴在高压下.
科学领域:
- 生物物理
- 细胞生物学
- 生物化学
背景情况:
- 生物分子凝聚物对于细胞功能至关重要,
- 液相分离 (LLPS) 是形成凝结物的关键物理机制.
- 功能障碍的凝聚物可能导致病态蛋白质聚合物.
研究的目的:
- 调查温度和压力对眼镜蛋白γ-晶体的LLPS的影响.
- 了解生物分子凝聚物的压力敏感性.
- 探讨奥斯莫利特在压力下稳定蛋白质凝结物的作用.
主要方法:
- 紫外/紫外和红外吸收光谱
- 光光谱学
- 光显微镜
- 介视相状态的表征
主要成果:
- γ-晶体LLPS对压力非常敏感,在低温下溶解在0.1kbar左右.
- 这种压力灵敏度明显高于典型的蛋白质展开 (~3 kbar).
- 发现三甲基胺- N- 氧化物 (TMAO) 增强了凝结的γ- 晶体滴的稳定性.
结论:
- 在高压环境中的生物必须适应生物分子凝聚物的压力敏感性.
- 像TMAO这样的度增加可能会给深海生物带来适应性优势.
- 了解压力对LLPS的影响与生命的起源和原细胞的形成有关.
相关概念视频
Molecular Comparison of Gases, Liquids, and Solids
54.5K
Particles in a solid are tightly packed together (fixed shape) and often arranged in a regular pattern; in a liquid, they are close together with no regular arrangement (no fixed shape); in a gas, they are far apart with no regular arrangement (no fixed shape). Particles in a solid vibrate about fixed positions (cannot flow) and do not generally move in relation to one another; in a liquid, they move past each other (can flow) but remain in essentially constant contact; in a gas, they move...
54.5K
Phase Diagrams
49.9K
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
49.9K
Rise of Liquid in a Capillary Tube
3.2K
When very thin cylindrical tubes, called capillaries, are dipped in a liquid, the liquid rises or falls in the tube compared to the surrounding liquid. This phenomenon is called capillary action. Capillary action occurs due to the combination of two opposing forces: the cohesive forces of the liquid, which cause it to stick to itself and form a rounded shape, and the adhesive forces between the liquid and the walls of the container, which cause the liquid to be attracted to the container walls.
3.2K
Deriving the Speed of Sound in a Liquid
931
As with waves on a string, the speed of sound or a mechanical wave in a fluid depends on the fluid's elastic modulus and inertia. The two relevant physical quantities are the bulk modulus and the density of the material. Indeed, it turns out that the relationship between speed and the bulk modulus and density in fluids is the same as that between the speed and the Young's modulus and density in solids.
The speed of sound in fluids can be derived by considering a mechanical wave...
The speed of sound in fluids can be derived by considering a mechanical wave...
931
High-Performance Liquid Chromatography: Introduction
3.4K
High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:
In HPLC, two phases play a critical role in the separation process:
3.4K
High-Performance Liquid Chromatography: Instrumentation
2.9K
High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
2.9K


