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相关概念视频

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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相关实验视频

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Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter
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Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter

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对"幽灵细胞计量"的评论

Dino Di Carlo1, Fumihito Arai2, Keisuke Goda3

  • 1Department of Bioengineering, University of California, Los Angeles, CA, USA. dicarlo@ucla.edu.

Science (New York, N.Y.)
|April 20, 2019
PubMed
概括
此摘要是机器生成的。

他和其他人. 开发了使用光学面具和时空转换的高速细胞成像技术. 然而,这项研究

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Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

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科学领域:

  • 生物光子学
  • 细胞成像
  • 流细胞测量

背景情况:

  • 对于生物研究来说, 流动细胞的高速成像是至关重要的.
  • 现有的方法经常在高率下遇到模糊和低信号噪声比.

研究的目的:

  • 评估Ota等人提出的方法和说法. 用于高速的细胞成像和分类.
  • 评估支持报告结果的实验数据是否足够.

主要方法:

  • 对细胞成像的光学面具和时空转换技术的分析.
  • 基于成像数据报告的细胞分类率的审查.

主要成果:

  • 这项由Ota等人进行的研究. 使用伪随机光学面具和时空转换.
  • 报道了高率,无模糊成像和高信号噪声比.
  • 据称每秒3000个细胞的细胞分类速度是基于成像数据的.

结论:

  • 由Ota等人提供的实验证据. 不足以证明他们报告的结论.
  • 需要进一步验证以支持高速细胞成像和分类能力的说法.