金属有机框架 纳米粒子辅助红细胞冷保存
Wei Zhu1, Jimin Guo1, Jacob Ongudi Agola1
1Center for Micro-Engineered Materials and the Department of Chemical and Biological Engineering , The University of New Mexico , Albuquerque , New Mexico 87131 , United States.
Journal of the American Chemical Society
|April 25, 2019
概括
基于的金属有机框架纳米颗粒提供了一种新的,无溶剂的红细胞冷保存方法. 这些纳米材料有效地抑制了冰晶的生长,与传统方法相比,改善了细胞恢复.
科学领域:
- 材料科学
- 生物技术
- 纳米技术
背景情况:
- 模仿抗蛋白的混合纳米材料对细胞和组织的冷保存有兴趣.
- 目前的冷保存方法通常依赖有毒的有机溶剂.
- 开发有效,无毒的冷保护剂对于保存生物样本至关重要.
研究的目的:
- 研究基于 (Zr) 的金属有机框架 (MOF) 纳米颗粒用于红细胞冷保存.
- 评估没有有机溶剂的MOF纳米颗粒的冰晶调制和冷保护性.
- 将MOF纳米颗粒与现有的冷保护剂的有效性进行比较.
主要方法:
- 基于Zr的五种不同性质的MOF纳米颗粒的合成和表征.
- 冰的再结晶抑制和冰的融化加速的评估,使用"斑点"测试.
- 使用MOF纳米颗粒对人类红细胞进行冷保存,并评估细胞恢复率.
主要成果:
- 基于Zr的MOF纳米颗粒显示出抑制冰的再结晶并加速冰的融化.
- 这些MOF纳米颗粒表现出极高的水稳定性和低血解活性.
- 使用MOF纳米颗粒的红细胞冷保存实现了回收率高达大约40%,超过基乙烯粉聚合物.
结论:
- 基于Zr的MOF纳米粒子代表了对红细胞冷保存的有希望的,无溶剂的方法.
- MOF纳米颗粒的独特表面化学和结构有助于冰晶的调节.
- 这项研究为设计用于更广泛的细胞和组织冷保存的MOF衍生纳米材料提供了基础.
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