相关实验视频
Updated: Aug 1, 2026

10:31
Real-time Live Imaging of T-cell Signaling Complex Formation
Published on: June 23, 2013
概括
T细胞激活可以通过超出CD3/T细胞受体综合体的途径发生. 研究表明,虽然Thy-1激活可以增加水平,但它需要CD3 / T细胞受体来产生互白素-2.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- T细胞激活对于适应性免疫至关重要.
- 建议使用其他T细胞激活途径,涉及CD2,Tp44,Thy-1,TAP和Ly-6等分子.
- Thy-1和CD3/T细胞受体 (TCR) 介导激活之间的关系需要澄清.
研究的目的:
- 为了研究基-1-依赖T细胞激活和CD3/TCR介导激活之间的关系.
- 为了确定Thy-1是否可以作为T细胞刺激的替代途径.
主要方法:
- 在CD3/TCR表达缺陷的Jurkat T细胞系的感染,与小鼠Thy-1.2基因.
- 用对Thy-1.2.2.的单克隆抗体对变质剂的治疗.
- 测量细胞质中自由 ([Ca2+]i) 的水平.
- 测试用于互白素-2 (IL-2) 生产.
- 补充有缺陷的Tiα或β链基因.
主要成果:
- 对Thy-1.2的单克隆抗体诱导了CD3/TCR阴性,Thy-1.2-阳性Jurkat转菌体中细胞质自由 ([Ca2+]i) 的增加.
- 这些Thy-1.2-激活细胞未能产生互白素-2 (IL-2).
- 通过替换缺陷的Tiα或β链基因来恢复CD3/TCR表达,恢复了表面CD3/TCR表达和对IL-2生产的Thy-1响应.
结论:
- 甲基-1介导的信号传递可以在T细胞中诱导流,而不依赖于CD3/TCR复合体.
- 然而,CD3/TCR复合体对于Ty-1-诱导的互白素-2产生至关重要.
- 这表明,虽然Thy-1可以启动信号事件,但CD3/TCR通路对于T细胞的完全激活至关重要,包括细胞因子的产生.
相关概念视频
Cell-mediated Immune Responses
Overview
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

