通过EFCAB9对生理学pH和的双重传感调节精子流动性
Jae Yeon Hwang1, Nadja Mannowetz2, Yongdeng Zhang3
1Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT 06510, USA.
Cell
|May 7, 2019
概括
EF-手结合域含蛋白9 (EFCAB9) 对于精子功能至关重要. 这种蛋白质作为pH值依赖的传感器,通过调节CatSper通道活动来调节精子运动和男性生育能力.
科学领域:
- 精子生物学 精子生物学
- 离子通道调节 离子通道调节
- 生殖生理学 生殖生理学
背景情况:
- 精子运动是由女性生殖道的不同pH值调节的.
- CatSper通道是精子过活化和男性生育能力的必不可少的pH敏感通道.
- 这些通道沿精子尾巴形成线性信号纳米域.
研究的目的:
- 为了确定参与CatSper通道调节的新型蛋白质.
- 阐明EF手结合域含蛋白9 (EFCAB9) 在精子功能中的作用.
- 了解EFCAB9调节CatSper通道活动和组织的机制.
主要方法:
- 利用淘汰赛小鼠模型研究EFCAB9功能.
- 研究了EFCAB9和CATSPERζ之间的相互作用.
- 分析了EFCAB9对CatSper通道活性和信号域的影响.
主要成果:
- EFCAB9被确定为一种双功能蛋白调节CatSper通道活动和域组织.
- EFCAB9,与CATSPERζ复合,对于pH依赖和对敏感的CatSper通道激活至关重要.
- 缺少EFCAB9会导致精子运动,生育能力受损,以及信号域组织中断.
- EFCAB9以依赖的方式与CATSPERζ直接相互作用,并在pH值升高时解离.
结论:
- EFCAB9作为一个细胞内,pH依赖的传感器.
- EFCAB9对于调节精子运动和男性生育能力至关重要.
- EFCAB9的机制涉及调节CatSper通道活动和信号域的空间组织.
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