低透性压力是血管积分缺陷的基础
Hung-Ji Tsai1, Anjali R Nelliat1,2, Mohammad Ikbal Choudhury3
1Center for Cell Dynamics, Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Nature
|May 10, 2019
概括
细胞形,或异常的染色体数量,导致细胞经历低透应激和血缺陷. 这会影响营养吸收和细胞大小,影响酵母和人体细胞.
科学领域:
- 细胞生物学
- 遗传学
- 分子生物学
背景情况:
- 染色体数异常的特征与癌症和出生缺陷等疾病有关.
- 虽然不同的形状会导致独特的基因表达和表型特征,但常见的细胞缺陷仍然不太清楚.
研究的目的:
- 鉴定常见的细胞缺陷和与形相关的基因表达特征.
- 在模型生物和人体细胞中研究形体的功能后果.
主要方法:
- 在芽酵母中利用一种策略,在多种体中平均转录组变化,减轻型特异性影响.
- 采用热力学建模来分析蛋白质不平衡,并预测皮质细胞大小关系.
- 进行全基因组查, 发现形细胞的依赖性.
主要成果:
- 鉴定出一种常见的形细胞基因表达特征,
- 在体酵母中观察到增加的血膜应激和受损的内细胞形成,这种缺陷也存在于人体体细胞中.
- 热力学建模证实了蛋白质组不平衡导致的低透性应激,并预测了观察到的平移细胞大小相关性.
- 发现形细胞依赖于营养物质运输物的泛素介导内细胞循环.
结论:
- 无质性通常会诱导类似低透的压力和血缺陷,影响内细胞和营养平衡.
- 这些细胞缺陷的根本驱动因素是形形成的蛋白质不平衡.
- 形细胞在营养运输物回收通路中表现出特殊的脆弱性,加剧了营养不平衡.
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