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通过抗克拉特林抗体抑制内细胞分裂的抑制

S J Doxsey, F M Brodsky, G S Blank

|July 31, 1987

概括

用抗体研究了对细胞运输至关重要的蛋白质克拉特林. 抑制克拉特林减少了内细胞分裂,但没有影响新合成的蛋白质的运输.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

克拉特林是一种细胞质蛋白质,已知与涂层坑和囊泡有关.
它在活细胞内的各种膜运输过程中的精确作用需要进一步阐明.

研究的目的:

为了研究克拉在细胞膜运输中的功能.
确定克拉特林在内分细胞和蛋白质贩运中的特定作用.

主要方法:

在活的CV-1细胞中引入单克隆抗克拉特林重链抗体.
对抗体引入对克拉斯林局部化和各种内细胞通路的影响的观察.

主要成果:

抗clathrin抗体导致了clathrin聚合,并减少了等离子膜上涂层坑的数量.
塞姆利基森林病毒的吸附性内细胞和液相内细胞减少了40%-50%.
新合成的流感血凝素的输送到血膜保持不变.

结论:

克拉特林在CV-1细胞的液相吸收和受体介导内细胞分裂中发挥着重要作用.
克拉特林在分泌途径内的构成性运输中并不必不可少.

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