聚合物"点击酶"加速小分子,蛋白质和细胞的铜点击反应
Junfeng Chen, Jiang Wang, Ke Li
1Institute for Molecular Engineering , The University of Chicago , Chicago , Illinois 60637 , United States.
Journal of the American Chemical Society
|May 25, 2019
概括
聚合物催化剂通过与蛋白质表面的可逆结合来意外地加速蛋白质的修饰. 这些含铜的纳米粒子在蛋白质和细胞表面上增强了类似酶的活性.
科学领域:
- 聚合物化学
- 生物催化
- 纳米技术
背景情况:
- 聚合物催化剂可以通过结合金属中心附近的基质来模仿金属酶.
- 使用铜 (CuI-SCNP) 的单链聚合物纳米粒子 (SCNP) 具有类似酶的催化作用.
- 之前的研究表明,SCNP在"吸收模式"中结合小分子.
研究的目的:
- 研究聚合物在加速蛋白质的催化改造中的意想不到的作用.
- 探索CuI-SCNP在蛋白质表面和细胞甘上的类似酶的催化能力.
- 在"附加模式"中阐明CuI-SCNP的结合和催化机制.
主要方法:
- 含铜的单链聚合物纳米粒子 (CuI-SCNP) 的合成和表征.
- 铜介导的亚酸循环添加反应的酶类催化研究.
- 对蛋白质表面和细胞甘氨酸的CuI-SCNP结合和催化的综合实验和计算分析.
主要成果:
- 在聚合物中的"吸收模式"中,CuI-SCNP展示了前所未有的催化速率.
- 与小分子催化剂相比,CuI-SCNP对修饰的蛋白质表面和细胞表面糖显著提高了点击反应效率.
- 这种增强的催化发生在"附着模式"中,SCNP通过多价和静电相互作用可逆地结合蛋白质表面.
结论:
- 聚合物催化剂,特别是CuI-SCNP,具有多价值,可逆和适应性结合蛋白质表面的新能力.
- 这种结合促进了蛋白质的加速催化改性,突出了它们作为人工金属酶的潜力.
- 这些发现表明聚合物催化剂在生物相关领域的应用范围更广.
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