长期的ex vivo血造干细胞扩张允许无条件的移植
Adam C Wilkinson1,2, Reiko Ishida3, Misako Kikuchi3
1Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, Stanford, CA, USA.
Nature
|May 31, 2019
概括
研究人员开发了一种新的培养系统,用于扩展功能性造血干细胞 (HSC). 这一突破有助于长期的HSC自我更新,并有助于改善HSC移植疗法.
科学领域:
- 血液学
- 干细胞生物学
- 复原医学
背景情况:
- 造血干细胞 (HSC) 在移植后对血液系统的再生至关重要.
- 目前的方法难以稳定出体HSC扩张,限制了治疗应用.
- 了解HSC微环境是改善ex vivo培养的关键.
研究的目的:
- 开发一种定义的无白蛋白培养系统,用于长期活体扩张功能性小鼠HSC.
- 在实验室中确定促进HSC自我更新的关键因素和条件.
- 评估扩大HSC在没有毒性预先调节的情况下进行移植的潜力.
主要方法:
- 系统优化培养条件,包括细胞因子水平和细胞外基因组件.
- 使用定义的无白蛋白介质,用聚乙烯醇取代血清白蛋白.
- 在接受者小鼠中评估了HSC扩张,自我更新能力和植入潜力.
主要成果:
- 在一个月内实现了236至899倍的功能性HSC扩张.
- 鉴定了高血栓形成素和低干细胞因子与纤维素对HSC自我更新的协同作用.
- 证明了扩展HSC的强大植入,而不需要有毒的预先调节.
结论:
- 开发了一种新的,定义的培养系统,使功能性HSC能够显著地在体外扩展.
- 该系统的效率和绕过毒性预先调节的潜力对HSC移植有重大影响.
- 这些发现促进了HSC基础研究和血液学中的临床应用.
相关概念视频
Induced Pluripotent Stem Cells
27.3K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.3K
Adult Stem Cells
33.4K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.4K
Embryonic Stem Cells
32.1K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.1K
Long-term Depression
33.2K
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
33.2K
Long-term Depression
3.1K
Long-term depression, or LTD, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTD is the process of synaptic weakening that occurs over time between pre and postsynaptic neuronal connections. The synaptic weakening of LTD works in opposition to synaptic strengthening by long-term potentiation (LTP) and together are the main mechanisms that underlie learning and memory.
Calcium Ion Concentration Mechanism
If over...
Calcium Ion Concentration Mechanism
If over...
3.1K
Heat and Free Expansion
2.8K
The work done by a thermodynamic system depends not only on the initial and final states but also on the intermediate states—that is, on the path. Like work, when heat is added to a thermodynamic system, it undergoes a change of state, and the state attained depends on the path from the initial state to the final state. Consider an ideal gas cylinder fitted with a piston. When the cylinder is heated at a constant temperature, the gas molecules absorb energy and expand slowly in a...
2.8K


