在炎症性肠道疾病中肠道微生物生态系统的多组学
Jason Lloyd-Price1,2, Cesar Arze2, Ashwin N Ananthakrishnan3
1Infectious Disease and Microbiome Program, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
炎症性肠道疾病 (IBD) 显示肠道微生物组功能失调,在活跃疾病期间细菌和分子中发生变化. 综合分析揭示了导致IBD失调的关键微生物,生化和宿主因素.
科学领域:
- 微生物学
- 胃肠病学
- 人类微生物组研究
背景情况:
- 包括克罗恩病和性结肠炎在内的炎症性肠病 (IBD) 影响全球数以百万计的人.
- IBD的特点是临床,免疫,分子,遗传和微生物的异质性.
- 之前的研究集中在导致IBD的个体因素上.
研究的目的:
- 提供IBD期间宿主和微生物活动的全面,综合,纵向视图.
- 描述与IBD活动相关的肠道微生物组的功能失调.
- 确定IBD发病过程中的关键微生物,生化和宿主因素.
主要方法:
- 在1年内对132名IBD患者进行长度研究.
- 从便,活检和血液样本生成综合分子概况 (共2965个).
- 宿主和微生物活动的分析,包括分类学,功能和生物化学概况.
主要成果:
- 在IBD爆发期间在肠道微生物群中表现出功能失调.
- 观察到选择性无气体的增加和强制性无气体的减少.
- 在微生物转录,代谢池和宿主抗体中发现了分子干扰.
结论:
- IBD 活动期间的特征是时间变化增加以及微生物组和宿主因子的明显变化.
- 综合性分析确定了导致IBD失调的核心微生物,生化和宿主因素.
- 这项研究提供了迄今为止最全面的IBD宿主微生物群相互作用描述,数据可通过IBD Multi'omics数据库获得.
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