抗菌鱼的结构和功能:胺的位置,膜插入的方向性和pH依赖的透性
Mihaela Mihailescu1, Mirco Sorci2, Jolita Seckute3
1Institute for Bioscience and Biotechnology Research , University of Maryland , Rockville , Maryland 20850 , United States.
Journal of the American Chemical Society
|May 31, 2019
概括
由于含有histidine,p1和p3表现出不同的膜相互作用. 皮西丁p1
科学领域:
- 生物物理
- 生物化学
- 膜生物学
背景情况:
- 皮西丁是富含希斯蒂丁的抗菌.
- 它们表现出两形状结构.
- 它们的pH活性表明生物医学潜力.
研究的目的:
- 调查胺变异如何影响胺透.
- 比较p1和p3的膜相互作用.
- 清除pH依赖的活性和膜插入.
主要方法:
- 中子衍射
- 染料泄漏试验
- 用NMR监测的定位
- 分子动力学模拟
- 电化学阻抗光谱学
主要成果:
- 与p1不同的是,p3的透性对pH敏感.
- 皮西丁p1表现出更深的膜插入和增加的膜破坏.
- 胺含量和两性决定了插入方向和pH弹性.
结论:
- 皮西丁中的胺变异导致差异膜透.
- 素p1的独特的胺 (H17) 赋予了抗pH的,更深层的膜插入.
- 提供了对抗微生物膜相互作用的机制见解.
相关概念视频
Structural Protein Function
29.8K
Structural proteins are a category of proteins responsible for functions ranging from cell shape and movement to providing support to major structures such as bones, cartilage, hair, and muscles. This group includes proteins such as collagen, actin, myosin, and keratin.
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
Collagen, the most abundant protein in mammals, is found throughout the body. In connective tissue, such as skin, ligaments, and tendons, it provides tensile strength and elasticity. In bones and teeth, it mineralizes to...
29.8K
Structural Protein Function
3.2K
3.2K
Porin Insertion in the Outer Mitochondrial Membrane
4.7K
Porins are beta-barrel proteins translocated to the mitochondrial outer membrane through the TOM complex into the intermembrane space. Porin precursors bind TIM chaperones within the intermembrane space and are guided to the Sorting and Assembly Machinery complex or SAM complex on the outer mitochondrial membrane.
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
Three models describe the assembly of porins by the SAM complex and their insertion into the outer membrane. Model 1 suggests that porins are assembled outside the SAM channel as the...
4.7K
Fruit Development, Structure, and Function
25.1K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.1K
Structure and Function of Erythrocytes
5.4K
There are between 4.2 and 6 million erythrocytes, also known as red blood cells, in every microliter of blood. These cells are small, flattened biconcave discs with centers that are depressed.
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
The erythrocyte plasma membrane is associated with proteins such as spectrin, which forms a flexible cytoplasmic meshwork. This meshwork allows erythrocytes to twist, turn, become cup-shaped, and regain their biconcave shape as they pass through narrow capillaries. Additionally, erythrocytes can form...
5.4K
Structure and Function of Platelets
3.2K
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
3.2K


