Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Habitat Fragmentation02:31

Habitat Fragmentation

21.2K
Habitat fragmentation describes the division of a more extensive, continuous habitat into smaller, discontinuous areas. Human activities such as land conversion, as well as slower geological processes leading to changes in the physical environment, are the two leading causes of habitat fragmentation. The fragmentation process typically follows the same steps: perforation, dissection, fragmentation, shrinkage, and attrition.
21.2K
Obesity01:24

Obesity

1.2K
The Body Mass Index (BMI) is a numerical value derived from a person's weight and height, used to categorize individuals into weight ranges. It is calculated using the formula: weight in kilograms divided by height in meters squared. Obesity is a health condition characterized by excessive accumulation of adipose tissue that poses health risks, often diagnosed with a BMI ≥ 30. This excess fat storage occurs when surplus dietary calories are converted into triglycerides and stored in...
1.2K
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

15.4K
The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
15.4K
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

4.1K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.1K
Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

9.0K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
9.0K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

18.7K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.7K
这页已由机器翻译。其他页面可能仍然显示为英文。View in English
  1. 首页
  2. 研究领域
  3. 生物医学和临床科学
  4. 瘤学和致癌症
  5. 预测和预后标志物
  6. 在肥胖症中与mff相互作用并促进线粒体碎片化
  1. 首页
  2. 研究领域
  3. 生物医学和临床科学
  4. 瘤学和致癌症
  5. 预测和预后标志物
  6. 在肥胖症中与mff相互作用并促进线粒体碎片化

相关实验视频

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

9.3K

在肥胖症中与Mff相互作用并促进线粒体碎片化

Philipp Hammerschmidt1, Daniela Ostkotte2, Hendrik Nolte3

  • 1Department of Neuronal Control of Metabolism, Max Planck Institute for Metabolism Research, Gleueler Strasse 50, 50931 Cologne, Germany; Center for Endocrinology, Diabetes and Preventive Medicine (CEDP), University Hospital Cologne, Kerpener Strasse 26, 50924 Cologne, Germany; Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and Center for Molecular Medicine Cologne (CMMC), University of Cologne.

Cell
|June 1, 2019

在PubMed 上查看摘要

概括
此摘要是机器生成的。

胺合成酶6 (CerS6) 产生C16:0脂,它们与线粒体裂变因子 (Mff) 结合,从而将肥胖与胰岛素抵抗联系起来. 抑制这种CerS6-Mff相互作用可以治疗代谢疾病.

更多相关视频

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.4K
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
07:55

Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment

Published on: September 25, 2017

8.3K

相关实验视频

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
08:53

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Published on: January 11, 2017

9.3K
Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.4K
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
07:55

Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment

Published on: September 25, 2017

8.3K

科学领域:

  • 生物化学
  • 细胞生物学
  • 代谢疾病

背景情况:

  • 宫外脂质沉积和线粒体动态变化与肥胖和胰岛素抵抗有关.
  • 连接这些过程的精确分子机制仍然不完全理解.

研究的目的:

  • 阐明胺合成酶 (CerS) 在脂代谢和线粒体功能中的作用.
  • 确定参与新陈代谢调节的特定C16:0脂及其相互作用伙伴.
  • 研究CerS6-Mff作为肥胖和胰岛素抵抗的潜在治疗点.

主要方法:

  • 在小鼠模型中利用了CerS5和CerS6的基因切除.
  • 通过生物化学测试研究了蛋白质-脂相互作用.
  • 在体外和体内评估线粒体形态和功能.
  • 在肥胖和胰岛素抵抗模型中分析了CerS6和Mff之间的遗传相互作用.

主要成果:

  • 缺少CerS6而不是CerS5可以防止肥胖和胰岛素抵抗.
  • 由CerS6衍生的C16:0脂特异地与线粒体裂变因子 (Mff) 结合.
  • 结合CerS6和Mff缺乏改善了肥胖引起的线粒体分裂和胰岛素抵抗.

结论:

  • 脂信号表现出酶特异性调节,CerS6在代谢平衡中发挥着关键作用.
  • 通过CerS6衍生的脂-Mff通路存在肝脏脂质积累,线粒体碎片化和胰岛素抵抗之间的直接机制联系.
  • 针对CerS6衍生的脂/Mff相互作用是代谢疾病的有希望的治疗策略.