细胞外矩阵受体的占用,整合素,是肌源性分化的一个控制点
1Department of Microbiology, University of Pennsylvania, Philadelphia 19104.
Cell
|October 9, 1987
概括
通过CSAT抗体阻断整合素功能,可以防止肉肌细胞分化. 移除抗体使神经细胞能够融合并形成肌肉细胞,突出显示整体蛋白.
科学领域:
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
- 肌肉的发展 肌肉的发展
背景情况:
- 肌体发生包括肌细胞增殖,融合和分化为多核肌管.
- 整合素是细胞表面受体,调解细胞-细胞外基质相互作用,在细胞粘附和信号传递中发挥作用.
- 整合素在启动肌原分化的特定作用需要进一步阐明.
研究的目的:
- 为了研究整合素功能在胚胎髓细胞的分化中的作用.
- 确定阻断整合素信号是否会影响肌细胞融合和肌肉特异性蛋白质的产生.
主要方法:
- 用CSAT治疗胚胎细胞,CSAT是一种单克隆抗体,向整合蛋白的β子单元.
- 细胞增殖,细胞融合和肌肉特异性蛋白质的表达 (desmin,meromyosin) 在抗体存在或不存在的情况下进行了评估.
- 通过去除CSAT抗体来观察分化恢复,进行了逆转实验.
主要成果:
- CSAT抗体治疗抑制了肌细胞融合和肌肉特异性美罗米素的产生,而肌细胞继续繁殖.
- 移除CSAT抗体很容易扭转分化中的阻断.
- 反向细胞表现出德斯和梅罗米奥辛的表达增加,并成功融合形成收缩的肌管.
结论:
- 肌细胞体和细胞外基质之间的因特林介导相互作用对于启动终端肌细胞分化至关重要.
- 集成蛋白信号传递是肌肉发育中从增殖转变为分化的一个关键调节器.
- 向整合素功能提供了一个控制肌肉细胞分化的潜在机制.
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