个体大脑器官可复制地形成人类大脑皮层的细胞多样性
Silvia Velasco1,2, Amanda J Kedaigle1,2,3, Sean K Simmons2,3
1Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Nature
|June 7, 2019
概括
人类大脑器官可以可靠地模拟大脑皮层的发育,显示一致的细胞类型生成和发育轨迹. 这项研究表明胚胎环境之外的中枢神经系统可以复制.
科学领域:
- 神经科学
- 发育生物学
- 干细胞研究
背景情况:
- 人类大脑有机体是理解大脑发育和疾病的关键实验模型.
- 与内源性脑组织相比,高的器官对器官变异性先前使其可复制性受到怀疑.
- 在胚胎形成之外的人类大脑发育过程的可复制性仍然是一个关键问题.
研究的目的:
- 在人背前大脑器官模型中评估细胞类型生成的可复制性.
- 确定人类大脑器官是否可以复制人类大脑皮层的细胞多样性和发育轨迹.
- 与内生脑组织相比,研究器官发育的变异性.
主要方法:
- 从干细胞系生成人类后脑前脑器官.
- 单细胞RNA测序分析来自21个单个细胞的166,000多个细胞.
- 对细胞类型汇编,发育轨迹和器官和内生脑组织的变异性进行比较分析.
主要成果:
- 95%的器官产生了高度一致和多样化的细胞类型, 反映了人类大脑皮层.
- 各个人的器官发育轨迹相似,变异性与内生大脑相似.
- 来自不同干细胞系的器官体表现出可再生细胞类型的产生.
结论:
- 复杂的中枢神经系统细胞多样性的可再生发展在胚胎环境之外是可以实现的.
- 在有机体中确定终端细胞身份是一个受约束的过程,从不同的干细胞来源和生长条件中始终出现.
- 人类大脑器官为研究大脑发育和疾病提供了可靠和可复制的平台.
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